STAT6基因多态性与哮喘的相关性对血浆IgE水平的影响  

Correlation between STAT ＋ 2964 gene polymorphism and asthma and its impact upon plasm IgE level

作　　者：李文[1] 陈敏[1] 黎东明[1] 吴斌[1] 

机构地区：[1]广东医学院附属医院呼吸内科,广东省湛江524001

出　　处：《中国基层医药》2007年第5期764-766,共3页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的 探讨STAT6基因＋2964G／A多态性与哮喘的相关性及对血浆IgE水平的影响。方法 用聚合酶链反应和限制性片段长度多态性（PCR／RFLP）方法检测哮喘组与对照组＋2964位点多态性，用酶联免疫吸附法（ELISA）测定血浆IgE水平。结果 ＋2964位点等位基因G、A频率在两组间分布的差异有统计学意义（Х^2=17．26，P〈0．01），等位基因G与哮喘关联，OR（G／A）=2．62，95％Cl=1．66～4．13，P〈0．01。两组基因型（GG、GA、AA）频率的分布差异亦有统计学意义（Х^2=19．52，P〈0．01），其优势比：OR（GG／AA）=3．30、95％Cl=1．26～8．66，P〈0．05；OR（GG／GA）=3．79，95％Cl=2．03～7．09，P〈0．01；OR（GA／AA）=0．869，95％Cl=0．303～2．496、P〉0．05；同组内，T等位基因携带者血浆IgE水平高于非携带者；同一基因型，哮喘组IgE水平高于对照组。结论 STAT6基因＋2964位点多态性是影响哮喘的重要候选基因，G等位基因与哮喘关联，并影响血浆IgE水平。Objective To investigate the correlation between ＋ 2964 locus polymorphism of STAT6 gene and the impact of the polymorphism upon plasm IgE level. Methods Polymerase chain reaction/restriction fragment length polymorphism（PCR/RFLP） techniquers were used to determine ＋ 2964 locus polymorphism in asthma and control group,and plasm IgE level were determined by ELISA. Results The frequency of allele G,A of ＋ 2964 locus had significant difference between two groups（Х^2 = 17.26, P 〈 0.01 ）, allele G was associated with asthma, OR （G/A） = 2.62,95 % Cl = 1.66- 4.13, P 〈 0.01. The genetype GG, GA and AA distribution was significantly different between two groups（Х^2 = 19.52, P 〈 0.01 ）, OR（GG/AA） = 3.30,95 % Cl = 1.26 - 8.66, P 〈 0.05 ; OR （ （GG/GA ） = 3.79,95 % Cl = 2.03 - 7.09, P 〈 0.01 ;OR （GA/AA） = 0. 869,95 % Cl = 0. 303 - 2. 496, P 〉 0.05. In each group, the plasm IgE level of subjects with G allele was higher than that of subjects without G allele, the plasm IgE level of asthma group was higher than that of control group with same genetype. Conclusion The ＋ 2964 polymorphism of IgE gene may be an important candidate gene for asthma. G allele is associated with asthma and increases the plasm IgE level.

关 键 词：哮喘 多态现象(遗传学) 免疫球蛋白E STAT6基因 

分 类 号：R5[医药卫生—内科学]