小单孢菌40027菌株噬菌体的分离及其生物学特性的研究  

Studies on the isolation and biological characterization of actinophages infecting Micromonospora sp. 40027

作　　者：李晓华[1] 周秀芬[2] 邓子新[2] 

机构地区：[1]中南民族大学生命科学学院国家民委生物技术重点实验室,武汉430074 [2]上海交通大学生命科学技术学院教育部微生物代谢重点实验室,上海200030

出　　处：《微生物学报》2007年第3期482-485,共4页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30570046);湖北省自然科学基金(2004ABA129);中南民族大学自然科学基金(YZZ04003)~~

摘　　要：以福堤霉素A产生菌--小单孢菌40027菌株为指示菌,从土壤中分离得到三株噬菌体：ΦHAU7、ΦHAU9和ΦHAU11.三株噬菌体的寄主专一性较强,在测试的15株放线菌菌株中,三株噬菌体能感染小单孢菌40027菌株和A-M-01菌株,ΦHAU9和ΦHAU11还能感染蔷薇小单孢菌（Micromonospora purprea）.三株噬菌体都是由多面体的头部和尾部组成;形成噬菌斑时培养基中适宜的Ca^2＋、Mg^2＋浓度分别为32mM和30mM;ΦHAU7在储存液中适宜的pH范围为6～12,而其它两株噬菌体的适宜的pH范围为6～10;在储存过程中三株噬菌体适宜的温度范围为28～37℃,经60℃保温30min后,除中ΦHAU7仍有53%活力之外,其它两株噬菌体全部失活.限制性内切酶酶切结果表明：三株噬菌体基因组DNA均为双链DNA;基因组大小分别约为60kb、58kb和55kb.高压脉冲电泳结果揭示：三株噬菌体基因组DNA均具有粘性末端.Three actinophages, ФHAU7 ,ФHAU9 and ФPHAU11, were isolated from soil using Micromonospora sp. 40027 as a indicator strain. Three phages showed very narrow host-ranges. Three phages could infect both Micromonospora sp. 40027 and Micromonospora A-M-01, ФHAU9 and ФHAU11 could also form plaques on Micromonospora purprea. All of the three phage particles have the hexagonal heads and tails; the plaque formation of three phages on Micromonospora sp. 40027 were best on DNA medium with addition of 32mM Ca^2＋ and 30mM Mg^2＋ ; ФPHAU7 was stable at pH 6 - 12, and other phages were stable at pH 6 - 10; the suitable incubation temperature for the propagation of three phages was between 28 ℃- 37℃ ; 53 % of ФPHAU7 remained viable and none of other phages was alive when they were incubated at 60℃ for 30min. Restriction digestion analysis of genomes of three phages indicated that they were all double-stranded DNA with cohesive ends, their genome size are ca. 60 kb,58 kb and 55kb, respectively.

关 键 词：小单孢菌40027菌株 噬菌体 福堤霉素A 

分 类 号：Q939.4[生物学—微生物学]