药用植物半枝莲的离体快繁  

Rapid Propagation in Vitro of Scutellaria barbata D.Don

作　　者：瞿大枞[1] 陈崇顺[1] 王轶[1] 杨燕燕[1] 曹伟杰[1] 

机构地区：[1]南京师范大学生命科学学院江苏省生物多样性与生物技术重点实验室,江苏南京210046

出　　处：《安徽农业科学》2007年第17期5065-5066,5095,共3页Journal of Anhui Agricultural Sciences

摘　　要：以茎尖为外植体进行了离体快繁研究。利用6-BA×IBA和6-BA×NAA两种激素组合诱导丛生芽,6-BA×IBA激素组合的最佳增殖培养基是MS+6-BA0.5 mg/L+IBA0.1 mg/L,增殖系数可达6.5;6-BA×NAA激素组合的最佳增殖培养基是MS+6-BA0.5 mg/L+NAA0mg/L,增殖系数可达6.1;两种组合无显著性差异。在MS基本培养基上即可生根且长势良好,生根率为100%;诱导丛生芽试验中6-BA浓度不能超过0.5 mg/L,否则会有愈伤组织出现。In order to protect the wild resource of Scutellaria barbara D.Don, its rapid propagation in vitro was studied using shoot-tip as explant. The results showed that the optimal mediums for the bud differentiation were MS ＋ 6-BA 0.5 mg/L＋ IBA 0.1 mg/L and MS＋6-BA 0.5 mg/L＋NAA 0 mg/L. Multiplication coefficients of the two were 6.5 and 6.1, respectively but there was no significant difference between them. MS medium was the optimal medium for root formation and the rooting rate was 100 %. Concentrations of 6-BA should be lower than 0.5 mg/L in order to avoid callus formation during shoot induction.

关 键 词：半枝莲 离体快繁 诱导 

分 类 号：Q943.1[生物学—植物学]