卷丹百合RAPD-PCR反应体系的正交优化  

Optimization of RAPD-PCR Reaction System of Lilium lancifolium Thunb.Using Orthogonal Design

作　　者：韩凌[1] 雷家军[1] 

机构地区：[1]沈阳农业大学园艺学院,辽宁沈阳110161

出　　处：《安徽农业科学》2007年第17期5087-5088,共2页Journal of Anhui Agricultural Sciences

摘　　要：以CTAB法提取卷丹百合的基因组DNA,采用正交试验对影响卷丹百合RAPD-PCR扩增的反应组分浓度进行优化。结果表明,最佳的RAPD-PCR反应体系(20μl)中含:10×buffer 2μl,模板DNA60 ng,Mg2+1.875 mmol/L,dNTP 0.8 mmol/L,引物30 ng及Taq酶0.5 U。Genomic DNA of Lilium lancifolium Thunb. was extracted by CTAB method. Factors in the system were studied in order to establish the optimum RAPD system of Lilium lancifolium Thunb. with orthogonal design. The results showed that a total volume of 20μl RAPD -PCR system consisted of 2 μl 10xbuffer, 60 ng template DNA, 1.875 mmol/L Mg^2＋, 0.8 mmol/L dNTP, 30 ng primer and 0.5 U Taq DNA polymerase was the optimum condition.

关 键 词：卷丹百合 RAPD 正交设计 体系优化 

分 类 号：Q94-33[生物学—植物学]