Inhibitory effect of 1,8-cineol (eucalyptol) on Egr-1 expression in lipopolysaccharide-stimulated THP-1 cells  

作　　者：Jian-ya ZHOU Xue-fen WANG Fa-di TANG Jian-ying ZHOU Guo-hua LU Yan WANG Ru-lian BIAN 

机构地区：[1]Department of Respiratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China [2]Zhejiang Respiratory Drugs Research Laboratory of State Drugs Administration of China, Zhejiang University, Hangzhou 310031, China

出　　处：《Acta Pharmacologica Sinica》2007年第6期908-912,共5页中国药理学报（英文版）

基　　金：the National Natural Science Foundation of China(№ 30670930);the Science and Technology Department of Zhejiang Province,China(№ 2004C23011)

摘　　要：Aim： To study the effects of 1,8-cineol （eucalyptol） on the expression of early growth response factor-1 （Egr-1） and NF-κB in the human monocyte THP-1 cell line stimulated by lipopolysaccharide （LPS）. Methods： The THP-1 cells were incu- bated with serial doses of 1,8-cineol （1, 10, and 100 mg/L, 30 min） before being stimulated with LPS （1 mg/L, 30 min）. The localization of Egr-1 in the THP-1 cells was detected by immunofluorescence and a laser scanning confocal microscope. The expression of Egr-1 in the nuclei and whole cell, and NF-κB in the nuclei, were measured by Western blot analysis. Results： When stimulated by LPS, the FITC- labeled Egr-1 was detected mainly in the nuclei. Moreover, the expression of Egr-1 in the whole cell increased markedly compared with the control cells. 1,8- Cineol pretreatment decreased the expression of Egr-1 in both the nuclei and whole cell of the LPS-stimulated THP- 1 cells, and this effect was concentration- dependent, but there was no reaction on the expression of NF-κB in the nuclei protein in the LPS-stimulated THP-1 cells. Conclusion： In a concentration-depen- dent manner, 1,8-Cineol reduces LPS-induced Egr-1 expression in nuclei and in whole cell of THP-1 cells, but shows no effect on NF-κB expression.Aim： To study the effects of 1,8-cineol （eucalyptol） on the expression of early growth response factor-1 （Egr-1） and NF-κB in the human monocyte THP-1 cell line stimulated by lipopolysaccharide （LPS）. Methods： The THP-1 cells were incu- bated with serial doses of 1,8-cineol （1, 10, and 100 mg/L, 30 min） before being stimulated with LPS （1 mg/L, 30 min）. The localization of Egr-1 in the THP-1 cells was detected by immunofluorescence and a laser scanning confocal microscope. The expression of Egr-1 in the nuclei and whole cell, and NF-κB in the nuclei, were measured by Western blot analysis. Results： When stimulated by LPS, the FITC- labeled Egr-1 was detected mainly in the nuclei. Moreover, the expression of Egr-1 in the whole cell increased markedly compared with the control cells. 1,8- Cineol pretreatment decreased the expression of Egr-1 in both the nuclei and whole cell of the LPS-stimulated THP- 1 cells, and this effect was concentration- dependent, but there was no reaction on the expression of NF-κB in the nuclei protein in the LPS-stimulated THP-1 cells. Conclusion： In a concentration-depen- dent manner, 1,8-Cineol reduces LPS-induced Egr-1 expression in nuclei and in whole cell of THP-1 cells, but shows no effect on NF-κB expression.

关 键 词：1 8-cineol EGR-1 THP-lcells NF-ΚB LIPOPOLYSACCHARIDE 

分 类 号：R97[医药卫生—药品]