人Man2c1转基因小鼠外周血中性粒细胞和CD8^+T细胞的调节  

Regulation of Peripheral Neutrophils and CD8^+T Lymphocytes in Human Man2c1-Transgenic Mice

作　　者：刘红霞[1] 苏卫[1] 向志光[1] 邸冉[1] 高珊[1] 朱立平[2] 张连峰[1] 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所中国医学科学院比较医学中心,北京100021 [2]中国医学科学院中国协和医科大学基础医学研究所,北京100005

出　　处：《中国实验动物学报》2007年第3期166-169,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：北京市转基因平台建设项目TC2006-02(20006303041231)

摘　　要：目的以转hMan2c1基因小鼠为模型,分析hMan2c1基因在转基因小鼠脾脏的蛋白表达和活性,研究hMan2c1基因对于机体免疫系统的影响。方法Western blot方法检测hMan2c1基因在脾脏的蛋白表达并检测小鼠脾脏α-甘露糖苷酶活性;血常规分析外周血中各种血细胞的比例;以BSA作为抗原观察机体的免疫应答;流式细胞技术观察外周血中CD4+、CD8+、B、NK细胞的数量。结果Western blot结果显示,与野生型小鼠比较,hMan2c1基因在转基因小鼠脾脏组织有明显的表达,α-甘露糖苷酶活性明显增高,中性粒细胞明显升高。BSA刺激后,转基因小鼠外周血免疫细胞中的CD8+T淋巴细胞明显高于野生型小鼠。结论hMan2c1基因在小鼠脾脏表达引起α-甘露糖苷酶活性显著升高,并进一步影响淋巴细胞的生成,增加中性粒细胞和CD8+T淋巴细胞对免疫原的应答。Objeetlve The hMan2cl gene encodes one of the α-mannosidases, hMan2cl gene transgenic mice were used to observe the protein expression and the enzymatic activity in spleen and the function of this gene on the development of lymphocytes. Methods The expression of hMan2cl was detected with Western blotting and the activity of the hMan2cl encoded protein was analyzed with enzymetic activity assay. The percentage of peripheral blood cells was detected with blood routine analysis and flow cytometry. Results Obvious expression of hMan2cl and enhancement of α-mannosidase activity was detected in the spleen tissues of transgenic mice compared with that in wild type mice. The percentages of white blood cells in the peripheral blood of the transgenic mice were significantly higher than those in the wild type mice （ P 〈 0.05 ） and the percentage of neutrophilic granulocyte was much higher than that in wild type control （ P 〈 0.05）. Significant increase of CD8^＋ T cells was also observed in the transgenic mice. Conclusion Our findings suggested that the expression of hMam2c gene in spleen can regulate the development of lymphocytes and enhance the response of CD8^＋ T cells to antigen stimulation.

关 键 词：α甘露糖苷酶 hMan2el 小鼠 转基因 免疫 

分 类 号：R349.64[医药卫生—基础医学]