用19个生化基因位点研究普通级Z:ZCLA长爪沙鼠的遗传多样性  

Genetic Diversity of Biochemical Markers of Z:ZCLA Mongolian Gerbils

作　　者：刘月环[1] 金晓音[1] 萨晓婴[1] 

机构地区：[1]浙江省医学科学院浙江省实验动物中心浙江省实验动物与安全性研究重点实验室,杭州310013

出　　处：《中国实验动物学报》2007年第3期208-211,I0005,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：浙江省医药卫生基金(编号:2001ZX0014)

摘　　要：目的：调查Z：ZCLA长爪沙鼠原种群普通级长爪沙鼠的遗传多样性。方法：用本实验室自行建立的长爪沙鼠19个生化基因位点的乙酸纤维素膜电泳技术并结合基本群体遗传学指标研究了普通级Z：ZCLA长爪沙鼠100个家系的遗传多态性。结果：Z：ZCLA长爪沙鼠在Es—1、Car-2、Hbb、Gpi-1、Cs-1、Ce-2、Idh-1、Mod—1呈单态，在Es-2、Es-3、Es-4、Es-6、Es-8、Es-9、Es-10、pd-1、gm-1、Trf、Akp-1个位点上呈现多态性，等位基因从2～4个不等，平均等位基因数3，0，平均杂合度0，512，平均多态信息量0．455。结论：提示目前本群遗传多样性水平处于中度多态。Objective To investigate the genetic diversity of the original population of Z： ZCLA Mongolian gerbils by biochemical markers. Methods 19 biochemical loci as markers were used to detect the genetic variation in the conventional closed colony of Z：ZCLA Mongolian gerbils. Results The loci Es-1, Car-2, Hbb, Gpi-1, Cs-1, Ce-2, Idh-1, Mod-1 had only one allele, while the loci Es-2, Es-3, Es-4, Es-6, Es-8, Es-9, Es-lO, Gpd-1, Pgm-l,Trf, Akp-1 had two to four alleles, respectively. The average heterozygosity, allele number and polymorphism information content were recorded as 0. 512, 3.0 and 0.455 respectively. Conclusion The analysis of polymorphism information content on 11 biochemical gene marker loci of this conventional＇closed colony of Z：ZCLA Mongolian gerbils revealed the genetic diversity at a moderate level.

关 键 词：Z:ZCLA长爪沙鼠 生化基因位点 遗传多态性 

分 类 号：R332[医药卫生—人体生理学]