竞争性逆转录聚合酶链反应定量检测对HCV　RNA  

Quantive detection of HCV RNA by competitive RT PCR

作　　者：苏秀芬[1] 牛俊奇[1] 胡玉琳[1] 张清泉[1] 许昶[1] 

机构地区：[1]白求恩医科大学一院

出　　处：《中华传染病杂志》1997年第1期32-34,共3页Chinese Journal of Infectious Diseases

摘　　要：为了解感染丙型肝炎病素（ＨＣＶ）后的（ＨＣＶＲＮＡ）含量与丙型肝炎病情轻重，预后，治疗反应的关系，采用竞争性逆转录聚合酶链反应（ＣＲＴ－ＰＣＲ）的方法，对９例感染了ＨＣＶ患者的血清进行了ＨＣＶＲＮＡ的定量检测。结果：６．６×１０３拷贝１毫升占８８．９％，６．６×１０４拷贝／毫升占１１．１％。结果提示：急慢性丙型肝炎及无症状ＨＣＶ感染者体内均含有较高的病毒滴度，且滴度低预后好，抗病毒疗效好。In order to determine the relationship between the amount of HCV RNA in sera from patients with HCV infection and severity of live disease prognosis and reations to therapy, HCV RNA was detected quantitatively in the sera from nine patients infected with HCV by competitive RT PCR. The results showed that the 6.6×10 3 copies of HCV RNA were found in 88.9% of these petients and 6.6×10 4/ml in 11.1%, respectively. This suggested that there was high tiler of HCV in both acute and chronic patients with viral hepatitis and asymptomic HCV carriers, and these patients with low titer of HCV RNA had good prognosis and reation to anti viral therapy.

关 键 词：丙型肝炎病毒 RNA 竞争性逆转录 PCR 

分 类 号：R512.63[医药卫生—内科学]