RP-HPLC测定双丹口服液中丹酚酸B、丹参素和原儿茶醛的含量  

Determination of contents of salvianolic acid B and danshensu and protocatechuic aldehyde of Shuangdan Oral Solution by RP-HPLC

作　　者：刘红亚[1] 雷勇[1] 杨大坚[2] 

机构地区：[1]四川省中药研究所,四川成都610041 [2]重庆中药研究院,重庆400065

出　　处：《中成药》2007年第6期839-842,共4页Chinese Traditional Patent Medicine

摘　　要：目的:建立双丹口服液(丹参、牡丹皮)中有效成分丹酚酸B、丹参素和原儿茶醛的HPLC含量测定方法。方法:采用Phenomenex LunaC18柱(4.6mm×150mm,5μm),甲醇-乙腈-甲酸-水(30:10:1:59)为流动相,检测波长:286nm,流速:1.0mL/min,测定丹酚酸B;甲醇-乙腈-2(v/v)甲酸溶液(9:4:87)为流动相,检测波长:281nm,流速:1.0mL/min,测定丹参素和原儿茶醛。结果:丹酚酸B平均回收率为98.6,RSD=1.91(n=6);丹参素平均回收率为99.2,RSD=2.2(n=6);原儿茶醛平均回收率为97.3,RSD=2.5(n=6)。结论:6批样品测定结果表明,该方法简便、准确,可用于双丹口服液丹酚酸B等3种有效成分含量测定。AIM ： To establish a method for determining the contents of salvianolic acid B, danshensu and protocatechuic aldehyde, the effective components of Shuangdan Oral Solution （Radix et Rhizoma Salviae miltiorrhizae, Cortex moutan） by HPLC. METHODS： Experiment was carried out on a Phenomenex Luna C18 column （4.6 mm × 150 mm,5 μm） with a mobile phase methanol-acetonitrile-methane acid-water（30： 10： 1：59） at a flow velocity of 1.0 mL/min and detection wavelength at 286 nm for salvianolic acid B, a mobile phase methanol-acetonitrile-methane acid water solution [ HCOOH： H2O （2：98 ） ] （9 ： 4：87 ） at a flow velocity of 1.0 mL/min and detection wavelength at 281 nm for danshensu and protocatechuic aldehyde. RESULTS： The average recoveries of salvianolic acid B,danshensu and protocatechuic aldehyde were 98.6% （RSD = 1.91% , n =6） ,99.2% （RSD =2.2% , n = 6） and 97.3% （RSD = 2.5% , n = 6） respectively. CONCLUSION： The results show that this method is easy to operate and accurate,and can be used to determine the contents of the three effective components in Shuangdan Oral Solution.

关 键 词：双丹口服液 丹酚酸B 丹参素 原儿茶醛 高效液相色谱 含量测定 

分 类 号：R927.2[医药卫生—药学]