中国汉族群体c-Ha-ras基因3′端-VNTR位点遗传多态性的初步研究  

POLYMORPHISM ANALYSIS OF THE c Ha ras 3′ VNTR LOCUS OF CHINESE HAN POPULATION WITH AFLP PAGE

作　　者：孙晨光[1] 伍新尧[1] 罗超权[1] 杨英浩[1] 贺奇才[1] 

机构地区：[1]中山医科大学生化教研室

出　　处：《中华医学遗传学杂志》1997年第1期21-23,共3页Chinese Journal of Medical Genetics

摘　　要：为了探讨中国汉族ｃ－Ｈａ－ｒａｓ基因３′端ＶＮＴＲ位点遗传多态性，用ＡＦＬＰ－ＰＡＧＥ法对１１２例无亲缘关系的健康个体进行了ｃ－Ｈａ－ｒａｓ基因３′端ＶＮＴＲ位点分析，共检出２１个等位基因，其片段大小分布范围在６３５～２６５１ｂｐ之间，基因频率分布在０．００４５～０．５９３８。父权排除率（Ｐｅ）＝０．４４４５；杂合度（Ｈ）＝０．６２５；个体识别力（Ｄｐ）＝０．８４４０。共发现３４种基因型，经χ２检验（χ２＝２３７．１１，ｄｆ＝２１０，Ｐ＝０．１０２５），符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡。家系调查表明这一位点的等位基因按孟德尔规律遗传。本遗传标记系统在人类学、法医学和医学遗传学的研究中有重要意义。Twenty one alleles of the c Ha ras 3′ VNTR locus were found in 112 unrelated healthy individuals of Chinese Han population by using AFLP PAGE. The frequencies of these alleles ranged from 0.0045 to 0.5938. The paternity exclusion probability of this locus was 0.4445. The heterozygosity was 0.6256. Its discriminating power was 0.8440. A total of 34 different genotypes were detected. The Chi square testing (χ 2=237.11, df =210, P =0.1025) statistically supported that these results were consistent with Hardy Weinberg's equilibrium. The investigation results of familial members showed that this genetic marker system was in line with Mendelian's law. The genetic marker system is of significance in anthropology, forensic medicine and medical genetics.

关 键 词：C-HA-RAS基因 3'端VNTR 遗传多态性 基因频率 

分 类 号：Q346.5[生物学—遗传学]