氧化型染发剂的遗传毒作用分析  

Study on Genotoxicity of Qxidized Hairdyes with SOS/Umu Genotoxicity Assay

作　　者：杨薇[1] 浦跃朴[2] 张徐军[2] 戴修道[1] 周德浩 

机构地区：[1]上海医科大学公共卫生学院,200025 [2]南京铁道医学院预防医学系,210009

出　　处：《预防医学情报杂志》1997年第1期10-13,共4页Journal of Preventive Medicine Information

摘　　要：为了探讨染发剂对DNA可能产生的损伤效应,采用SOS/Umu试验对氧化型染发剂的主要成分对苯二胺(PPD)和过氧化氢(H_2O_2)的遗传毒性进行了观察。结果表明:在无S_9存在的条件下,PPD在375～6000μg/管的剂量范围内与酶诱导率之间无剂量反应关系,H_2O_2在4～125μg/管的剂量范围内与酶诱导率之间有剂量反应关系(r=0.995,p<0.01),PPD被H_2O_2氧化后则显示出了遗传毒作用(r=0.988,p<0.01);4种氧化型染发剂(F、G、S及X牌)的1剂(主要含PPD)在39～1250μg/管的剂量范围内与酶诱导率之间无剂量反应关系,2剂(主要含H_2O_2)在9.8～312.5μg/管剂量范围内与酶诱导率之间有剂量反应关系(r_F=0.950,P<0.01;r_G=0.926,P<0.01;r_S=0.997,P<0.01;rx=0.993,P<0.01)。1剂和2剂按实用比例等量混和后在9.8～312.5μg/管(按PPD浓度计)剂量范围内有剂量反应关系(r_F=0.994,P<0.01;r_G=0.932,P<0.01;r_S=0.998,P<0.01;r_X=0.987,P<0.01)。在S_9存在的条件下,待测物在受试剂量范围内均未显示出遗传毒作用。结果提示,氧化型染发剂有DNA损伤作用,且其致突变性与氧化剂H_2O_2的作用有关。The genotoxieity of the main gradient of oxidized hairdyes (phenylenediamine) and its oxidant (hydrogen peroxide) was studied with SOS/Umu assay. It showed that purifed phenylenediamine (PPD) did not damage DNA, H_2O_2 and oxidized PPD caused DNA damage arid had dose-response relationship (r_(H_2O_2)=0.995, p<0.010; r_(PPD+H_2O_2)=0.988, P<0.01). It indicated that oxidized PPD had direct genotoxieity and the mutagenesis of it resulted from H_2O_2 by comparison the regrssion coefficients of them. The study of 4 oxidized hairdyes also confirmed this conclusion with SOS/Umu assay. Consequently, when we study the mutation of oxidized hairdyes, we should pay more attention to the DNA-damaging of H_2O_2 itself.

关 键 词：氧化型 染发剂 对苯二胺 过氧化氢 遗传毒性 

分 类 号：TQ658.34[化学工程—精细化工] R114[医药卫生—卫生毒理学]