血清法监测人工感染弓形虫病猪特异抗体的比较  

Comparison of Different Methods to Detect the Specific Antibody in Swines Infected with Toxoplasma gondii

作　　者：江涛[1] 何会时 龚大春[3] 陈声国[1] 聂浩[1] 姚宝安[4] 赵俊龙[1] 

机构地区：[1]华中农业大学动物医学院 [2]荆州市畜牧局,湖北荆州434100 [3]长江大学动物科学学院,湖北荆州434025 [4]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《湖北农业科学》2007年第3期427-430,共4页Hubei Agricultural Sciences

基　　金：湖北省科技攻关计划项目(2006AA205A02)

摘　　要：应用ELISA、LAT、IHA血清试验对8头人工感染弓形虫猪的血清抗体滴度变化规律进行了监测,并对不同检测方法的结果进行了比较。结果发现,试验猪在皮下感染弓形虫RH株速殖子后,血清中的特异性抗体首次检出的时间方面,LAT(第4d)早于ELISA(第14d)和IHA(第20d);检出持续时间方面,LAT(54d)长于ELISA(44d)和IHA(19d);平均检出率方面,LAT(7/8)高于ELISA(6.7/8)和IHA(5/8)。试验结果表明,ELISA和LAT适合于猪弓形虫病的诊断和血清学调查,LAT更适合于猪弓形虫病的早期检测。Observation and comparison of the dynamic changes of specific antibody were done by detecting antibody titer in sera of swines inoculated hypodermically the tachyzoites of Toxoplasma gondii RH strain by ELISA, LAT and IHA. The special antibodies in the sera of the infected swine were detected primarily on the day 14th by ELISA, 4th by LAT and 20th IHA post-infection. The detected continual time of LAT and ELISA was 54 days and 44 days respectively, longer than that of IHA （19 days） and the average detected rate of them was also higher than that of IHA. The results showed that ELISA and LAT were suitable for investigating serology and diagnosis of swine toxoplasmosis, and the latter was more suitable for diagnosis of toxoplasmosis in initial stage of the infection.

关 键 词：龚地弓形虫 rMIC3 ELISA LAT IHA 

分 类 号：S858.28[农业科学—临床兽医学]