多聚左旋赖氨酸促进大鼠肢芽细胞软骨分化的研究  

Research of Poly-L-Lysine on Rat Limb Bud Cell Chondrogenic Differentiation in vitro

作　　者：纪影畅[1] 李宇[1] 李宏生[1] 许宏权[1] 蔡湘娜[1] 

机构地区：[1]汕头大学医学院第一附属医院整形科,广东汕头515041

出　　处：《汕头大学医学院学报》2007年第2期86-87,96,F0003,共4页Journal of Shantou University Medical College

摘　　要：目的:探讨多聚左旋赖氨酸(PL)对大鼠肢芽细胞向软骨细胞分化的促进作用及其可能机制。方法:取大鼠肢芽细胞在含不同剂量PL的培养液中培养4 d,根据培养细胞所形成的软骨集落形成情况、与阿尔新蓝结合量和Ⅱ型胶原的表达情况,判断PL促进肢芽细胞向软骨细胞分化现象;根据不同时间添加PL来推断其发挥最大作用的时间;用免疫组化检测神经钙粘附蛋白的表达。结果:PL促进培养中大鼠肢芽细胞软骨集落的形成的最佳剂量可能为10μg/mL;PL发挥最大作用的时间为培养的第24 h内;免疫组化显示:5μg/mL组中Ⅱ型胶原和神经钙粘附蛋白的表达增强。结论:PL在一定浓度范围内以剂量依赖性方式促进大鼠肢芽细胞向软骨细胞分化且可能是通过增强神经钙粘附蛋白的表达来完成的。Objective： To study the promotion effect and possible mechanism of poly-L-lysine（PL）on rat limb bud cell chondrogenic differentiation in vitro. Methods： High density cultures of rat limb bud cell were exposed to culture media containing PL and cultured for four days. Chondrogenesis was assayed based on cartilage nodule number, Alcian blue bound（OD600）and expression of type Ⅱ collagen. By adding PL to the cultures at different time, the best time point of PL was inferred. The neural cadherin expression was examined by immunohistochemistry. Results： The best concentration of PL might be 10 μg/mL. Chondrogenesis stimulated by PL mainly happened on first 24 hours. The immunohisto- chemistry showed that the expressions of type Ⅱ collagen and neural cadherin were increased. Conclusion： PL can promote rat limb bud cell chondrogenic differentiation in vitro, which may be achieved by enhancing the expression of neural cadherin.

关 键 词：多聚赖氨酸 软骨形成 钙粘附蛋白 细胞培养 

分 类 号：Q813.1[生物学—生物工程] Q954.65