DMSO体外杀螨效果及机制的实验观察  

Experimental observation on the in vitro killing effect of Dimethyl sulfoxide (DMSO) on human mite Demodex and its mechanism of action

作　　者：赵亚娥[1] 郭娜[1] 张灵小[1] 

机构地区：[1]西安交通大学医学院,西安710061

出　　处：《中国人兽共患病学报》2007年第6期608-610,613,共4页Chinese Journal of Zoonoses

基　　金：陕西省自然科学基金(2006C247);陕西省卫生厅基金(06D29)资助

摘　　要：目的探讨二甲基亚砜(DMSO)体外杀灭人体蠕形螨的效果及药物作用机制。方法采用透明胶带粘贴法获取螨虫,在MoticDM图像采集软件系统下,动态观察并拍摄不同浓度二甲基亚砜对离体螨虫的体外杀螨效果以及药物作用前后蠕形螨形态和活动度的变化。结果二甲基亚砜对两种人体蠕形螨的杀虫作用与空白对照组比较均有显著性差异(P<0.05),杀虫效果与药物浓度和作用时间呈正比,40%的二甲基亚砜是体外杀螨的最低有效浓度。显微镜下观察发现,加入二甲基亚砜后,虫体收缩变形,活动加剧,经历一段时间的兴奋期后,虫体活动逐渐缓慢,颜色逐渐变淡,透明死亡。与临床常用10%和5%的硫磺乳相比,二甲基亚砜体外杀螨的效果优于硫磺乳(P<0.05)。结论二甲基亚砜具有较强的体外抗蠕形螨作用,其作用机制可能是触杀、神经毒、失水共同作用而使螨虫死亡。To investigate the in vitro killing effect of Dimethyl sufoxide （DMSO） on human mite Dcmodex and it mechanism of action, mites were collected with adhesive cellophane tape technique, the classical microscopic counting method was used to determine the killing effect of DMSO on mites and the dynamic observations were pictured with Motic Image DM soft- ware. It was demonstrated that significant difference in the in vitro killing effect of DMSO existed between the DMSO-treated group and the control group （P〈0.05）. The killing effect positively correlated with concentration of drug and time of action. , and the lowest effective concentration of DMSO to express its killing effect was 40%. Additionally, DMSO showed a better killing activity than 10%0 or 5 % Lac sulfur （P〈0.05）. After treatment with DMSO, mites were contracted and twisted with accelerated activity for a certain period of time, then their activities gradually slow down with the body turned to be transparent and finally they die. In conclusion, it is clear that DMSO has a powerful killing effect on human mite Demodex in vitro, whose mechanism of action might be due to the combined actions of contact killing, neuromuscular toxic action and deprivation of body fluids.

关 键 词：人体蠕形螨 二甲基亚砜 杀螨率 作用机制 

分 类 号：R384[医药卫生—医学寄生虫学]