番茄LeNCED1基因5′-侧翼序列克隆及LeNCED1p:GUS融合基因构建  

5′-FLANKING SEQUENCE CLONING OF LeNCED1 GENE IN TOMATO AND ITS FUSION WITH β-GLUCURONIDASE GENE

作　　者：万小荣[1] 李玲[2] 

机构地区：[1]仲恺农业技术学院生命科学学院,广东广州510225 [2]华南师范大学生命科学学院,广东广州510631

出　　处：《核农学报》2007年第3期232-236,共5页Journal of Nuclear Agricultural Sciences

基　　金：广东省自然科学基金博士科研启动基金(06301202);仲恺农业技术学院博士启动基金(G2360225);广东省自然科学基金(06025049)

摘　　要：从番茄基因组中克隆LeNCED1基因5′-侧翼1456 bp调控序列,经生物信息学分析表明,LeNCED1基因上游调控序列中存在响应昼夜节律的顺式作用元件Circadian、光响应元件Box I、逆境胁迫响应元件(TC-rich repeats)、ABA响应元件ABRE等,构建了LeNCED1基因上游调控序列与报告基因GUS融合的植物双元表达载体pLeNCED1p:GUS。The cloning of 1456-bp 5′-flanking sequence of LeNCED 1 gene from tomato and its fusion with β-glucuronidase gene （GUS） were reported. The flanking sequence was bioinformatically analyzed in the database of plant cis-acting regulatory element （PlantCARE）. The result showed that there were several important cis-acting elements, including Circadian （response to circadian rhythm）, Box I （response to light）, TC-rich repeats （response to stress） and ABRE （response to ABA）, in the upstream regulatory region of LeNCED1 gene. The binary vector harboring 5′-flanking sequence of LeNCED1 gene fused with GUS gene originated from pCAMBIA1301 was constructed to be used in coming plant transgene.

关 键 词：番茄 NCED基因 5′-侧翼序列克隆 融合基因构建 

分 类 号：S641.2[农业科学—蔬菜学]