猪胰岛素前体氨基酸位点突变对其结构和折叠的影响  

The Effect of the Mutant of Porcine Insulin Precursor on Its Folding and Structure

作　　者：赵杰[1] 许根俊[1] 冯佑民[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2007年第4期475-478,共4页Journal of Zhejiang Sci-Tech University(Natural Sciences)

摘　　要：蛋白质的氨基酸序列决定了它的高级结构和生物功能。用蛋白质工程技术得到了猪胰岛素前体(PIP)的突变体[B10D]PIP,并得到HPLC纯的蛋白质样品。通过对该突变体的研究发现:它的结构稳定性、二硫键稳定性、体外再折叠速率和效率都与野生型PIP有所不同。The three-dimensional structure and biological function of a protein is uniquely determined by its amino acids sequence, [B10D-]PIP is obtained by the means of protein engineering and purified by HPLC. [B10D-]PIP is studied and the results indicate that the structure stability, disulfide stability, and in vitro refolding efficiency of [B10D]PIP are different from that of native PIP.

关 键 词：胰岛素 猪胰岛素前体 稳定性 再折叠 

分 类 号：Q512.2[生物学—生物化学]