胰岛素和胰岛素样生长因子对人成骨样细胞MG63骨钙素基因表达的影响  

The effects of Insulin and IGF-1 on expression of bone gla protein of human osteoblast-like cell line MG63

作　　者：马艳芬[1] 李万根[1] 陈澍[1] 

机构地区：[1]首都医科大学大兴医院内分泌科,广州510260

出　　处：《中国骨质疏松杂志》2007年第6期398-401,共4页Chinese Journal of Osteoporosis

基　　金：广东省自然科学基金项目(000333)

摘　　要：目的在一定条件下对人成骨样细胞MG63进行体外培养,并用胰岛素、胰岛素样生长因子-1(Insulin like growth factor-1,IGF-1)进行干预,以17-β雌二醇做阳性对照。观察药物干预对MG63细胞骨钙素基因表达的影响。为糖尿病性骨质疏松的治疗提供理论和实验依据。方法用DMEM培养基培养MG63细胞,并以胰岛素、IGF-1、雌二醇进行干预。抽提各样本总RNA后,行逆转录并对骨钙素的cDNA进行荧光扩增和定量。结果各药物组内基因拷贝数差异均有统计学意义(P<0.05),各组药效呈浓度依赖性增加趋势。在本实验所选的药物浓度范围内,各药物组的最高药效浓度均为所用的最高药物浓度,分别为200nmol/L、100nmol/L、1000nmol/L。3组药物的骨钙素拷贝数增长率比较差异有统计学意义(P<0.05),胰岛素组的拷贝数增长率明显高于雌二醇组和IGF1组(P<0.05),IGF-1与雌二醇组拷贝数增长率比较差异无统计学意义(P>0.05)。结论胰岛素、IGF-1和雌二醇一样,均有显著促进MG63分化的作用。Objective To investigate the effect of insulin and insulin like growth factor-1 （IGF-1 ） on the express of bone gla protein （osteocalcin）of osteoblast-like cell line MG63, 17-β estradiol were used as the contrast medicine. This experiment focuses on exploring the pathogenesis of the osteoporosis and providing the experimental basis for the treatment of the osteoporosis of diabetes. Methods The MG63 cells（10S/mL） were seeded into the DMEM medium. Then the cultured MG63 were exposed to insulin, IGF-1 and 17-β estradiol for 48 hours with 0.1% bovine serum albumin phenol-free DMEM. The total RNA of every sample was extracted and then do reverse transcriptions to gate the cDNA chains of genes of bone gla protein. Fluorescence real time quantitative PCR assay was carried out to examine the mRNA expression of bone gla protein. The nest primers were used in the PGR assay. The statistics of results were performed by software of SPSS 11.0. ANOVA and post hoc statistical analyses were used to determine differences among treatments （ P 〈 0.05 ）. Results The expression of mRNA copys for bone gla protein was significantly different inside the groups of Insulin, IGF-1 and 17-β estradiol （ P 〈 0.05）. The effect of every medicine increased dose-dependently. The best medical effect concentrations of Insulin, IGF-1 and 17-βestradiol is the highest medical effect concentrations of every group （200 nM ,100 nM ,1000 nM respectively） in this experiment. The increase rates of bone gla protein mRNA copy of Insulin, IGF-1 and 17-βestradiol were dramatically different（ P 〈 0.05）. The increase rate of Insulin group was significantly higher than that of IGF-1 and 17-β estradiol groups（ P 〈 0.05）and there were no significant difference between IGF-1 and 17-β estradiol（ P 〉 0.05）. Conclusion like 17-βestradiol, insulin and IGF-1 improved the differentiation function of MG63.

关 键 词：胰岛素 IGF-1 MG63细胞 分化 

分 类 号：R587.2[医药卫生—内分泌]