健脾方对去势大鼠维生素D代谢的影响  

The Effects of JianPi prescription on Vitamin D_3 metabolism in ovariectomized rats

作　　者：王翔[1] 赵咏芳[1] 石印玉[1] 詹红生[1] 曹月龙[1] 徐宇[1] 

机构地区：[1]上海中医药大学附属曙光医院伤骨科,上海200032

出　　处：《中国骨质疏松杂志》2007年第6期429-432,413,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金资助项目(30371793;30500674);上海市重点学科资助项目(T0303);教育部博士点基金资助项目(20040268012);上海市启明星计划资助项目(04QMX1434)

摘　　要：目的采用放射免疫方法与分子生物学方法从VitD代谢的角度探讨健脾方防治原发性骨质疏松症的机制。方法建立卵巢切除致骨质疏松大鼠模型,运用骨组织病理学、血液生物化学等方法综合观察健脾方对模型大鼠骨密度、尿吡啶酚(PYD)和血清骨钙素(BGP)的影响;同时应用放射免疫方法与逆转录聚合酶链式反应观察健脾方对模型大鼠血清1,25(OH)2D3和小肠、肾脏组织维生素D受体基因(VDR mRNA)表达的影响。结果健脾方能升高模型大鼠的骨密度,降低模型大鼠血清1,25(OH)2D3的含量(P<0.05),同时能上调模型大鼠小肠、肾脏VDR mRNA的表达。结论健脾方能增强机体对血清1,25(OH)2D3反应敏感性,上调小肠与肾脏VDR mRNA的表达,调节VitD代谢。Objective To study the effects of JianPi prescription on vitamin D3 metabolism in ovariectomized rats. Methods Rats were randomly divided into four groups： Sham group, OVX group, JianPi prescription therapy group and lalpha （OH）-vitamin D3 therapy group （OVX ＋ lalpha （OH）-vitamin D3 ）. Ten rats were in respective group. The time point of administering rats drug was seven days after OVX operation, and the drug was stopped after three months. Bone mineral density was determined, serum 1,25-Dihydroxyvitamin D3 and the expression of the vitamin D receptor （VDR） mRNA were also analyzed. Results （1） 1 alpha （OH）-vitamin D3 increased BMD of the OVX rats significantly （P 〈 0.05）, respectively vs OVX control group. （2） lalpha （OH）-vitamin D3 significantly decreased serum 1,25-Dihydroxyvitamin D3 level compared with OVX group （P 〈 0.05）. （3） lalpha （OH）-vitamin D3 may upregulate expression of VDR mRNA of the intestine and kidney. Conclusions lalpha （OH）-vitamin D may protect the ovariectomized rats from bone loss. These results demonstrated that lalpha （OH）- vitamin D could prevent and treat the primary osteoporosis through modulating the VD metabolism.

关 键 词：中药 骨质疏松症 维生素D 

分 类 号：R971.6[医药卫生—药品]