复方六月雪对HepG2.2.15细胞HBsAg和HBeAg的抑制作用  

The inhibitory effect of compound Liuyuexue on HBsAg and HBeAg in the HepG2.2.15 cells

作　　者：张士军[1] 黄春喜[1] 谢海源[1] 黄仁彬[1] 林军[1] 

机构地区：[1]广西医科大学药理学教研室,广西南宁530021

出　　处：《中国医院药学杂志》2007年第6期715-717,共3页Chinese Journal of Hospital Pharmacy

基　　金：广西科学研究与技术开发计划项目(编号:桂科攻0322024-5E)

摘　　要：目的:观察复方六月雪(CLYX)体外抗乙型肝炎病毒(HBV)的作用。方法:采用四甲基噻唑蓝(MTT)法检测CLYX对HepG2.2.15细胞的半数毒性浓度(TC50)和最大无毒浓度(TC0);在TC0基础上观察不同浓度药物作用于HepG2.2.15细胞,分别在第72h和144h收集细胞培养上清液,采用酶联免疫吸附实验(ELISA)法测定上清液HBsAg和HBeAg的滴度。结果:TC50为3.070g·L-1,TC0为0.945g·L-1,复方六月雪对HepG2.2.15细胞毒性较低。无毒浓度的复方六月雪在HepG2.2.15细胞培养中可有效地抑制细胞HBsAg(乙型肝炎表面抗原)和HBeAg(乙型肝炎E抗原)的分泌;且治疗指数(TI)均大于2,为高效低毒的抗HBV药物。结论:CLYX在体外有显著的抗HBV的作用,且毒性较低。OBJECTIVE To study the inhibitory effect of traditional Chinese medicine compound Liuyuexue （CLYX） on HBV in vitro. METHODS The TC50 and TG0 of CLYX to the HepG2. 2. 15 cells were determined by MTT assay. At non-cellulotoxic concentration, the different concentrations of CLYX were added respectively to HepG2. 2. 15 cells, which were incubated subsequently. At 72 h and 144 h after treatment with drugs, the culture media were collected for determining the levels of HBsAg and HBeAg by ELISA. RKSULTS TC50 = 3. 070 g.L^-1 , TG0 = 0. 945 g·L^-1. CLYX had little cellulotoxicity. CLYX at the largest non-cellulotoxic concentration could suppress HBsAg and HBeAg expression in HepG 2.2. 15 cell, and the therapeutic index （TI） 〉2. CLYX is an effective and low toxic anti-HBV drug. CONCLUSION CLYX can inhibit significantly HBV in vitro, and is a lower toxic drug.

关 键 词：复方六月雪 HepG2.2.15细胞培养 HBsAg HBeAg 

分 类 号：R285.6[医药卫生—中药学]