高效液相色谱法测定大鼠血浆和肝脏匀浆中甲基莲心碱浓度  

Determination of neferine by HPLC in rat plasma and liver

作　　者：彭芳[1] 黄颖[1] 李帅[1] 胡本容[1] 王嘉陵[1] 向继洲[1] 

机构地区：[1]华中科技大学同济医学院药理系,湖北武汉430030

出　　处：《中国医院药学杂志》2007年第6期762-764,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的:建立测定大鼠血浆和肝脏匀浆中甲基莲心碱浓度的高效液相色谱法。方法:大鼠血浆和肝脏匀浆样品经乙醚萃取,进样分析。采用HypersilBDSC18(4.0mm×250mm,5μm)柱分离。以甲醇-磷酸二氢钾缓冲液-三乙胺(71∶29∶0.002)为流动相,检测波长为282nm。结果:血浆中甲基莲心碱的线性范围为31.25μg·L-1～2.00mg·L-1;日内和日间RSD分别为小于8.0%和5.0%,绝对回收率为77.45%～89.23%。其在肝脏匀浆中的线性范围为62.5μg·L-1～16.0mg·L-1,日内和日间RSD均小于3.0%,绝对回收率为73.53%～88.43%。结论:该方法符合生物样品的检测要求,可应用于大鼠血浆和肝脏匀浆中甲基莲心碱浓度的测定。OBJECTIVE To develop an HPLC method for the determination of Neferine（NEF） in rat plasma and liver. METHODS The samples were separated on a reversed phase column （ Hypersil BDS C18, particle size 5 μm, 4. 0 mm × 250 mm） after extracting by ether. The mobile phase consisted of methanol phosphate buffered saline triethylamine （71 ： 29 ： 0. 002）, and the UV detection wavelength was 282 nm. RESULTS The linear range was 31.25 μg·L^-1 - 2. 00 mg·L^-1 in plasma, and 62. 5 μg·L^-1- 16.0 mg·L^-1 in liver. The absolute recovery rate was between 77. 45% and 89. 23% in plasma, and between 73. 53% and 88. 43% in liver. It showed good reproducibility that the precision was within 8. 0% in plasma, and within 3. 0% in liver. CONCLUSION This method accorded with the standard of drug analysis in biological specimen and was successfully applied to determining the concentration of NEF in rat plasma and liver.

关 键 词：甲基莲心碱 高效液相色谱法 血浆浓度 肝脏匀浆浓度 

分 类 号：R927.2[医药卫生—药学]