视黄酸诱导蛋白16蛋白质结构功能分析  

Analysis on the protein structure and function of retinoic acid induced 16

作　　者：王阁[1] 邓婧[1] 杨进[1] 郑继军[1] 王红中[1] 胡庆[1] 张志敏[1] 陈川[1] 王东[1] 李增鹏[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所肿瘤中心,重庆市400042 [2]第三军医大学大坪医院野战外科研究所病理科,重庆市400042

出　　处：《世界华人消化杂志》2007年第12期1342-1346,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金;No.30570410;No.30370341;全国优秀博士专项基金资助项目;No.200261~~

摘　　要：目的:分析Tec激酶区作用蛋白视黄酸诱导蛋白16(RAI16)的蛋白质结构和功能信息.方法:利用相应的计算机软件及数据库,对RAI16 cDNA序列进行分析和比较,并对编码的蛋白质氨基酸序列及其二级结构进行分析和预测.结果:RAI16 cDNA序列由2863个核苷酸组成,编码759氨基酸蛋白质,RAI16蛋白包括一段由31个氨基酸组成的信号肽,没有跨膜区,亚细胞定位于内质网上,有多种二级结构形式,为一紧密包裹的球状蛋白质,是一种分泌蛋白,有多个磷酸化和蛋白酶切位点.RAI16蛋白为不稳定蛋白,不稳定系数为53.11.疏水性GRAVY(grand average of hydropathicity)值为-0.136.结论:RAI16蛋白作为一种新的胞内信号蛋白,很可能在肝癌细胞的诱导分化信号转导转录中与Tec协同发挥重要作用.AIM： To analyze the protein structure and function of Homo sapiens retinoic acid induced 16 （RAIl6） cDNA nucleotides sequence. METHODS： Using some related computer software and database, RAIl6 cDNA sequence and the deduced amino acid （AA） sequence were analyzed and compared with those of other species, and the secondary structure and hydrophobicity of the protein were determined and predicted. RESULTS： RAIl6 cDNA sequence consisted of 2863 nucleotides encoding a 759-AA protein. RAIl6 protein included a 31-AA signal peptide. The protein had no transmembrane domain, and its subcellular localization was in endoplasmic reticulum. The secondary structure contained several kinds of conformations. RAIl6 protein appeared as a compact and globular domain, and was a secretory and hydrophilicity protein （grand average of hydropathicity： -0.136）. The protein was also unstable （the unstable coefficient： 53.11）, with many phosphorylation and proteinase sites. CONCLUSION： As a new signal protein, RAIl6 may be involved in the signal transduction and transcription of hepatoma cell differentiation by interacting with Tec protein.

关 键 词：Tec蛋白 视黄酸诱导蛋白16 核苷酸 蛋白质结构 

分 类 号：R341[医药卫生—基础医学]