IL-10基因启动子区-592A/C位点多态性与HBV感染转归的关系  

Genetic Polymorphisms of Interleukin-10 Gene Promoter-592A/C Site with Hepatitis B Virus Infection

作　　者：张健[1] 李卫宁[1] 林华[1] 

机构地区：[1]北京大学深圳医院检验科,广东深圳518036

出　　处：《现代检验医学杂志》2007年第3期81-84,共4页Journal of Modern Laboratory Medicine

摘　　要：目的探讨汉族人白细胞介素-10(IL-10)基因启动子区-592位点单核苷酸多态性与乙型肝炎病毒(HBV)感染、转归的关系。方法采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法,检测231例HBV感染者,165例HBV感染康复者和135例正常对照者IL-10基因启动子区-592A/C位点基因型。结果IL-10基因启动子区-592A/C位点基因型和等位基因在HBV感染组、HBV感染康复组和正常对照组之间的分布频率比较差异无显著性(P>0.05),在血清HBV-DHA<1×103copies/ml的HBV感染者组和HBV-DHA≥1×103copies/ml组之间的分布频率比较差异亦无显著性(P>0.05);但IL-10基因启动子区-592A/C位点AA基因型和A等位基因在慢性乙型肝炎组出现的频率明显高于HBV无症状携带组,两组之间分布比较差异有显著性(P<0.05)。结论汉族人IL-10基因启动子区-592A/C位点多态性可能与人群对HBV易感性及感染后的病毒血症水平无显著相关性,但该位点基因多态性与HBV感染后的肝脏炎症反应有关。Objective To investigate the correlation between polymorphisms of interleukin-10（IL-10） gene promoter region 592A/C site and the susceptibility and clinical phenotypes of hepatitis B virus （HBV） in Han population. Methods Using polymerase chain reaction with restriction fragment length polymorphism （PCR-RFLP） method ,the single nucleotide polymorphisms （SNP） of the promoter region of IL-10 gene at position-592A/C were detected in 231 patients with chronic hepatitis B, 165 individuals spontaneously recovered from HBV infection and 135 normal controls. Results No significant difference was found in frequencies of genotypes and alleles of IL-10 gene promoter region at position-592A/C among normal controls,individuals spontaneously recovered from HBV infection and patients with chronic hepatitis B （P〉0. 05）,also between patients with HBV infection with HBV-DNA〈1×10^3 copies/ml and those with HBV-DNA≥1 ×10^3 copies/ml （P〉0. 05）. However,frequencies of AA genotype and allele A at posltion-592A/C in chronic hepatitis B were significantly higher than that in asymptomatic HBVcarriers （P〈0.05）. Conclusion It is probable that genetic polymorphisms of interleukin-10 promoter region 592A/C site are not associated with both susceptibility of HBV infection and HBV-DNA replication after infected HBV in Chinese Han population,but are related to inflammatory reaction in the liver of the patients with HBV infection.

关 键 词：白细胞介素-10 基因多态性 乙型肝炎病毒 

分 类 号：Q346.5[生物学—遗传学] R512.62[医药卫生—内科学]