氯化钴预处理对缺氧预适应小鼠海马组织HIF-1α表达的影响  

The Effects of CoCl_2 Pretreatment on HIF-1α Expression in Hippocampus of Hypoxic Preconditioned Mice

作　　者：邵国[1] 高翠英[1] 吕国蔚[1] 

机构地区：[1]首都医科大学神经生物学系

出　　处：《首都医科大学学报》2007年第3期337-340,共4页Journal of Capital Medical University

基　　金：国家自然科学基金(39670271);北京市自然科学基金(7962009)资助项目~~

摘　　要：目的检测氯化钴预处理后小鼠海马组织缺氧诱导因子-1α(HIF-1α)在急性重复氧过程中的变化及其可能的机制。方法用数字表法将Balb/C小鼠随机分为氯化钴实验组和生理盐水对照组,2组动物分别在实验前3 h注射氯化钴和生理盐水,进行0次(H0)、1次(H1)、4次(H4)缺氧暴露,随即分别取海马组织,用Western blot及EMSA法检测HIF-1α蛋白质含量及HIF-1 DNA结合活性。结果生理盐水预处理后,小鼠的缺氧耐受时间逐次显著递增;氯化钴预处理后缺氧耐受时间恒定增高。氯化钴预处理H0、H1、H4组海马组织的HIF-1α蛋白质含量差异未见统计学意义,但HIF-1 DNA结合活性在H1组升高、H4组降低。生理盐水预处理组海马组织的蛋白质含量及HIF-1 DNA结合活性在H0、H1、H4组均依次增加。结论氯化钴预处理能显著增强小鼠对缺氧的耐受能力、激活海马组织中的HIF-1,但其分子机制可能与急性重复缺氧暴露增强缺氧耐受力的机制不同。Objective To investigate the effect of CoCl2 pretreatment on expression of HIF-1α in the hippocampus of hypoxic preconditioned mice. Methods Balb/c mice were randomly divided into group of CoCl2 and normal saline （NS） , respectively injected with CoCl2 and NS 3 h before exposure to hypoxia for 0 run（H0） , 1 run（ H1 ） and 4 runs（H4）. The tissue of hippocampus was removed immediately after the exposure. Technique of Western blot and electrophoretic mobility shift assay（EMSA） were used to measure the HIF-1α protein level and HIF-1α DNA binding activity, respectively. Results The hypoxic tolerance time in group NS was significantly increased run by run but unchanged in group CoCl2. In group CoCl2 , the level of HIF-1α protein was unchanged whereas HIF-1α DNA binding activity was increased in H1 and decreased in H4. In group NS, both HIF-1α protein and DNA binding activity were increased. Conclusion Hypoxic-tolerance of mice to hypoxia and HIF-1α in their hippocampus are, respectively, increased and activated by CoCl2 while its underlying molecular mechanisms are thought to be different from those induced by acute repetitive hypoxic exposure.

关 键 词：氯化钴 缺氧诱导因子-1 海马 小鼠 

分 类 号：R338.26[医药卫生—人体生理学]