反相高效液相色谱法测定不同品种升麻中27-脱氧升麻亭的含量  

Determination of 27-deoxyactein in various specimens of Rhizoma Cimicifugae by RP-HPLC

作　　者：潘瑞乐[1] 陈迪华[1] 斯建勇[1] 赵晓宏[1] 朱占军[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094

出　　处：《中南药学》2007年第3期206-208,共3页Central South Pharmacy

基　　金：国家科技部"十五"重大科技攻关项目(2001BA701A55-13);国家自然基金重点项目(3050860)资助

摘　　要：目的采用高效液相色谱法测定不同品种、不同产地升麻样品中27-脱氧升麻亭的含量。方法色谱柱为YMC-Pack ODS-C18柱（4.6 mm×150 mm,5μm）;流动相：乙腈-水-乙酸（40∶60∶1）,流速0.6 mL.min-1,柱温26℃,示差检测器。结果27-脱氧升麻亭在2.588-20.7μg有良好的线性,相关系数r=0.999 7,平均加样回收率97.5%,RSD=2.75%（n=5）。结论方法简便,结果准确,可为升麻药材质量评价提供参考。Objective To determine the content of 27-deoxyactein in various specimens of Rhizoma Cimicifugae by RPHPLC. Methods The YMC-Pack ODS-C18 column （4.6 min× 150 mm, 5 μm） was used. The mobile phrase consisted of acetonitrile-water-acid （40 ： 60 ： 1）. The flow rate was 0.6 mL · min^-1. The column temperature was 26 ℃. The refractive index detector was used. Results The linear range was 2. 588-20. 7 μg （r=0. 999 7）. The average recovery was 97.5% with RSD of 2.75% （n=5）. Conclusion This method is simple and easy to perform and the resuits are reliable. It provides a reference standard for the quality control of Rhizoma Cimicifugae.

关 键 词：升麻 升麻属 27-脱氧升麻亭 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]