肺气肿肺气虚证模型大鼠免疫功能状态的实验研究  

Experimental study on the immune function in rat models with pulmonary emphysema of pulmonary qi-deficiency syndrome

作　　者：刘向国[1] 方志斌[1] 蔡圣荣[1] 王元勋[1] 

机构地区：[1]安徽中医学院,安徽合肥230038

出　　处：《甘肃中医学院学报》2007年第3期9-12,共4页Journal of Gansu College of Traditional Chinese Medicine

基　　金：安徽省自然科学基金项目(00044118);安徽省教育厅自然科学基金资助项目(2003KJ234)

摘　　要：目的观察肺气肿肺气虚证模型大鼠血清、支气管肺泡灌洗液(BALF)及肺组织中白细胞介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)含量的动态变化,探讨其在肺气肿肺气虚证发病中的作用机制。方法将60只SD大鼠随机分为对照组和模型1月、2月、3月组,采用气管内注入脂多糖(LPS)和烟熏双因素复合制作肺气肿肺气虚证大鼠模型,采用放射免疫法检测血清、BALF、肺组织中IL-10、TNF-α的含量。结果与对照组相比,模型组大鼠血清、BALF和肺组织中IL-10的含量显著降低,TNF-α的含量显著升高(P<0.05或P<0.01)。结论IL-10、TNF-α通过对免疫调节和炎性反应的影响,参与肺气肿肺气虚证发病过程,可能是肺气肿肺气虚证发生和发展的重要因素之一。Objective ：To observe the contents change of IL-10 and TNF-α in serum, BALF and pulmonary tissue in rat models with pulmonary emphysema of pulmonary qi-deficiency syndrome, and study their significance and probable mechanism as well. Methods：60 SD rats were randomly divided into four groups：the control group and the model group（ which were randomly divided into three model subgroups of one month, two months and three months）. The rat models with pulmonary emphysema of pulmonary qi-deficiency syndrome were established by endotracheal instillation of lipopolysaccharide （LPS）twice, exposure to cigarette smoking. The contents of IL-10 and TNF-α in serum, BALF and pulmonary tissue were measured by the RIA method. Results： The contents of IL-10 in serum, BALF and pulmonary tissue were significantly reduced, but the contents of TNF-α in serum, BALF and pulmonary tissue were significantly elevated in model group of each month compared with those of healthy control group （P 〈 0. 05 or P 〈 0. 01）. Conclusions：IL-10 and TNF-α participate in the course of pulmonary emphysema of pulmonary qi-deficiency syndrome by the effects on immunoregulation and inflammatory reaction, they could be one of important factors happening and developing pulmonary emphysema of pulmonary qi-deficiency syndrome.

关 键 词：肺气肿 肺气虚证 白细胞介素-10 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]