高效液相色谱法测定栽培射干中次野鸢尾黄素的含量  

Determination of Irisflorerntin in Cultivated Belamcanda chinensis(L.) DC by HPLC

作　　者：陈银华[1] 孟军华 侯俊杰[1] 刘合刚[1] 

机构地区：[1]湖北中医学院药学院,湖北武汉430065 [2]湖北省武汉市第三医院,湖北武汉430061

出　　处：《时珍国医国药》2007年第6期1391-1392,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的应用高效液相色谱法测定栽培射干中次野鸢尾黄素的含量,考察其药材的内在质量。方法采用高效液相色谱法,色谱柱为TSK ge1 ODS 80 TS250 mm×4.6 mm;流动相为甲醇-磷酸水溶液（pH=3）∶53%∶47%;流速为1 ml/min;检测波长为266 nm;柱温35℃。结果次野鸢尾黄素在0.0118-0.118μg范围内与色谱峰面积呈线性关系（r=0.9999）;平均加样回收率为99.84%,RSD为2.059%,次野鸢尾黄素的含量为0.1252%。结论该法简便、准确、灵敏度高,栽培射干中的次野鸢尾黄素的含量大于0.10%,符合《中国药典》要求。Objective To determine irisflorentin in cultivated Belarncanda chinensis（ L. ）DC by HPLC, and to inspect the quality of its ingredients. Methods Using HPLC with TSK gel ODS Column80（250 mm ×4.6 mm） ,the mobile phase was composed of a mixture of methanol - phosphoric aicd aqueous soluton （ pH = 3 ） ： 53% ： 47%, the flow rate was 1 ml/min, the UV detection wavelength was set at 265 nm,and the temperature was at 35℃. Results Irisflorentin showed linear relationship with peak areas in the range of 0.0118 -0.118 μg（ r = 0. 999 9 ）. The average recovery was 99.84% , RSD was 2.509%. Conclusion The method is simple,rapid and accurate. The quantity of irisflorentin in Cultivated Belamcanda Chinensis （L.）DC exceeds 0.10% and meets the Chinese Pharmacopoeia.

关 键 词：高效液相色谱法 射干 次野鸢尾黄素 

分 类 号：R284.1[医药卫生—中药学]