反相高效液相色谱法测定半枝莲中原儿茶酸的含量  

RP-HPLC Determination of Protocatechuic Acid in Scutellaria barbara

作　　者：陈香爱[1] 袁志芳[1] 田亚平[1] 李德强[1] 张兰桐[1] 

机构地区：[1]河北医科大学药学院,河北石家庄050017

出　　处：《时珍国医国药》2007年第6期1405-1406,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立半枝莲药材中原儿茶酸的含量测定方法。方法样品用30%的乙醇回流提取,提取液滤过,以反相高效液相色谱（RP-HPLC）法测定。用DiamonsilTMC18色谱柱（250 mm×4.6 mm,5μm）,以甲醇-0.05%磷酸为流动相,流速为1.0 ml·min^1,检测波长为258 nm。结果原儿茶酸的保留时间约为7 min,且与其它峰的分离度大于1.5。原儿茶酸的线性范围为0.018-0.184μg（r=0.999 8）,最低检测限为3 ng,平均回收率和RSD分别为99.7%和0.27%。结论该方法简便快速,结果准确可靠,可为半枝莲药材的质量评价提供有效手段。Objective To establish a method to determine the content of protoeatechuic acid in Scutellaria barbara. Methods The sample was extracted with 30% EtOH under reflux, and then the extract solution was filtered. RP - HPLC was used for the determination of protocatechuic acid in Scutellaria barbata. The chromatographic procedure was carried out using DiamonsilTM C18 as an analytic column and a mixture of 25 volume of methanol, 75 volume of 0.05% phosphoric acid as a mobile phase at a flow rate of 1.0 ml·min^-1 The detection wavelength was set at 258 nm. Results The peak of protocatechuic acid appeared for about 7 minutes. Resolution of protocatechuic acid was more than 1.5 and its linear range was between 0. 018 and 0. 184 μg （ r = 0. 999 8 ）. The minimum limit of detection was 3 ng. The average recovery and RSD value of protocatechuic acid were 99.7% and 0.27%, respectively. Conclusion This method is sensitive and quick and can be used for the quality control of Scutellaria barbara.

关 键 词：反相高效液相色谱法 半枝莲 原儿茶酸 含量测定 

分 类 号：R284.1[医药卫生—中药学]