不同地区栗蚕(Dictyoploca japonica)的RAPD分析  

Analysis of the Dictyoploca japonica With RAPD Technique

作　　者：曹兰娟[1] 杨宝山[2] 李俊[1] 李敏[1] 王卓[1] 杨瑞生[1] 秦利[1] 

机构地区：[1]沈阳农业大学生物科技学院,沈阳110161 [2]济南大学,济南250022

出　　处：《蚕业科学》2007年第2期293-296,共4页ACTA SERICOLOGICA SINICA

摘　　要：利用RAPD技术对10个地区的栗蚕(Dictyoploca japonica)基因组DNA进行多态性分析,以期查明我国栗蚕种质资源的分布及种群间的亲缘关系。运用11个引物进行扩增,共扩增出123条清晰稳定的条带,其中有96个位点具有多态性,多态性位点比率为78.05%。遗传距离(D)在0.178 9～0.414 6之间。通过聚类分析,7个东北地区的栗蚕聚在一起,重庆巫溪和广西南宁地区的栗蚕聚在一起,湖北恩施地区的栗蚕自成一类。研究结果表明,RAPD标记具有简便、快速的优点,可用于栗蚕的鉴定和遗传分析。RAPD （random amplified polymorphic DNA） molecular marker technique was used to analyze the genomic DNA of Dictyoploca japonica from 10 different areas in order to find the relationship between the position of Dictyoploca japonica specie sources in our country and population. Eleven ISSR primers were used to amplify the genomic DNA, 123 distinguishable bands were produced, among which 96 bands showed polymorphism. The percentage of polymorphism was 78.05%. The relative distance value among different Dictyoploca japonica races were 0. 178 9 -0. 414 6. The result of clustering analysis revealed that Dictyoploca japonica from 7 regions in Northeast was clustered into one group, Dictyoploca japonica from Wuxi of Chongqing and Nanning of Guangxi was clustered into other group, and Dictyoploca japonica from of Enshi of Hubei showed obvious genetic diversity to the others.

关 键 词：栗蚕 随机扩增多态性DNA 分子标记 遗传距离 

分 类 号：S885.9[农业科学—特种经济动物饲养]