重组鸡痘病毒对豚鼠的生物安全性  

Biosafety of Recombinant Fowlpox Virus to Guinea Pigs

作　　者：金明兰[1] 金宁一[2] 鲁会军[2] 马鸣潇[1] 郑敏[1] 刘慧娟[1] 李旭[1] 计越[1] 霍小伟[1] 唐树勋 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]军事医学科学院十一所全军基因工程重点实验室,长春130062 [3]吉林省通化市畜牧兽医总站,通化134001

出　　处：《中国生物制品学杂志》2007年第6期422-425,共4页Chinese Journal of Biologicals

基　　金："863"计划项目(2001AA213071);吉林省科技厅重大项目(20040202).

摘　　要：目的检测重组鸡痘病毒在妊娠豚鼠及其子代豚鼠体内的安全性。方法重组鸡痘病毒以正常剂量和超大剂量同时免疫妊娠豚鼠和子代豚鼠,通过临床观察、病理组织学、PCR等方法,检测重组鸡痘病毒对妊娠豚鼠的毒性和子代豚鼠生长的影响以及子代豚鼠的病毒DNA残留。结果临床观察和病理组织学检测表明在整个实验期间,免疫重组鸡痘病毒的妊娠豚鼠精神状态、饮水、采食等临床表现一切正常,未表现出临床症状和不良反应,未造成早产、流产、死胎、弱胎;用正常和超大剂量免疫重组鸡痘病毒的子代豚鼠均未出现任何临床症状,内脏器官未见明显的病理变化,且未检测到病毒DNA。结论构建的重组鸡痘病毒生物安全性好,对妊娠豚鼠和子代豚鼠无安全威胁。Objective To study the biosafety of recombinant fowlpox to pregnant guinea pigs and their offspring. Methods Immunize pregnant guinea pigs and their offspring with recombinant fowlpox virus at normal and large dosages separately. Analyze the virulence of recombinant fowlpox virus to pregnant guinea pigs as well as the influence on growth of offspring by clinical observation and histopathological method, and determine the residual viral DNA in offspring by PCR. Results During clinical observation,the pregnant guinea pigs showed no clinical symptoms or adverse reactions, and their mental status ,water drinking and food taking were normal. The immuniztion with recombinant fowlpox virus caused no premature delivery, abortion, fetal death or ambly-fetus. The offspring showed no any chnical symptoms or physiological change in organs, and no viral DNA was detected. Conclusion The constructed recombinant fowlpox virus showed good biosafety to pregnant guinea pigs and their offspring.

关 键 词：重组鸡痘病毒 生物安全性 妊娠豚鼠 子代 

分 类 号：R373.9[医药卫生—病原生物学]