转海蓬子Na^+/H^+逆向运输蛋白基因番茄的耐盐性研究  

RESEARCH ON SALT-TOLERANCE OF TOMATO TRANSFORMED BY NHAP GENE FROM SALICORNIA BIGELOVII TORR

作　　者：张桂和[1] 郭建春[2] 叶妙水[2] 

机构地区：[1]海南大学海洋学院,海南海口570228 [2]中国热带农业科学院热带作物生物技术国家重点实验室,海南海口571101

出　　处：《贵州科学》2007年第2期47-50,77,共5页Guizhou Science

基　　金：海南省教育厅科研基金重点项目(HjKj200403);海南省自然科学基金(30406);中国热带农业科学院自然科学基金(Rky0526)

摘　　要：以转海蓬子Na+/H+逆向运输蛋白基因(Nhap)的番茄(Lycopersicon esculentum Mill.)阳性植株和对照植株为材料,测定叶片相对含水量、质膜透性、K+/Na+比值和叶绿素含量4项耐盐指标.结果表明:在盐胁迫条件下,转基因植株的相对含水量、K+/Na+比值和叶绿素含量明显高于对照植株,而叶片质膜透性明显低于对照植株,说明Nhap基因已经在番茄转化植株体内表达,提高了转基因植株的耐盐性.Tomato Plants transformed by Nhap （Na^＋/H^＋ antiporter） gene from Salicornia Bigelovii Torr and control plants were tested using several salt - tolerance index such as relative leaf water content, plasma membrane permeability , ratio of K^＋/Na^＋ and content of chlorophyll. The results show that the transgenic tomato plants have higher relative leaf water content, ratio of K ^＋/Na ^＋ and chlorophyll content, and lower plasma membrane permeability than control plants under salt - stress conditions, which proves that Nhap gene has functioned in transgenie tomato plants and their salt - tolerance has been increased.

关 键 词：Nhap基因 转基因番茄 耐盐指标 

分 类 号：Q343.12[生物学—遗传学]