抗凝活性蛋白产生菌T.albuminosus的诱变育种  

Mutation breeding of Termitomyces albuminosus producing anti-coagulation protein TA-P

作　　者：杨洁晶[1] 钱秀萍[1] 赵凤生[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《工业微生物》2007年第3期33-36,共4页Industrial Microbiology

摘　　要：真菌Termitomyces albuminosusTA-SD能产生具有抗凝活性的蛋白质TA-P。本实验采用紫外诱变法对TA-SD菌株进行诱变育种,筛选高活性的突变株。出发菌株TA-SD紫外照射40s时,正突变率和抗凝活性提高的幅度最大,分别为33.3%和104.7%。N型突变株和放线菌酮抗性突变株的正突变率和活性提高的幅度均大于S型突变株和非抗性突变株。经筛选突变株SD-A-8的抗凝活性比出发菌株提高了2.3倍。该突变株传代稳定。The fungus, Termitomyces albuminosus TA-SD could produce anti-coagulation protein TA-P. In order to screening the mutants with higher activities, the mutation breeding of the strain TA-SD was carried out by ultraviolet mutagenesis. When the time of ultraviolet radiation was 40 seconds, the most positive mutation rate and anti-coagulation activity were reached, and it was 33.3 % and 104.7 % respectively. Both the positive mutation rate and anti-coagulation activity in the N-type mutants and cycloheximide-resistant mutants were higher than it in the S-type mutants and non- cycloheximide-resistant mutants. After screening the mutant SD-A- 8 was obtained and its anti-coagulation activity was 2.3 times higher than the initial strain. The mutant was stable after the transfer of culture.

关 键 词：TERMITOMYCES albuminosus 抗凝活性 诱变育种 

分 类 号：TQ460.1[化学工程—制药化工]