日本黄姑鱼与鮸状黄姑鱼的分子标记和遗传变异  

Molecular Genetic Markers and Variation of Cultured Nibea japonica and Nibea miichthioides

作　　者：柴学军[1] 胡则辉[1] 徐君卓[1] 

机构地区：[1]浙江省海洋水产研究所,浙江舟山316100

出　　处：《宁波大学学报（理工版）》2007年第2期174-178,共5页Journal of Ningbo University：Natural Science and Engineering Edition

基　　金：国家"十五"科技攻关项目(2001BA505BO202)

摘　　要：利用RAPD技术对日本黄姑鱼(Nibea japonica)和鮸状黄姑鱼(Nibea miichthioides)养殖群体进行了基因组DNA遗传分析.从120个随机引物中,筛选出19个扩增效果好,条带清晰的引物进行扩增.结果表明:(1)日本黄姑鱼和状黄姑鱼群体内个体间的平均遗传相似度分别为0.885 3和0.890 1;用SPSS软件对群体内个体间遗传相似度的方差分析结果表明,在鮸状黄姑鱼群体内存在显著差异(P<0.05),而在日本黄姑鱼群体内则差异不显著,表明日本黄姑鱼的种质较单一,存在近交衰退的危险;(2)2种黄姑鱼之间相似度为0.7850,遗传距离为0.2150,由此判断2种黄姑鱼为同一属的2个不同物种,这与形态学分析的结果相一致.As important economic marine fish, Nibea japonica and Nibea miichthioides are similar in morphological characters, which are easily influenced by the geographical regions and environments. Therefore, it is often difficult to distinguish them accurately at morphological level, and then investigating the genetic differences becomes necessary. Among many identifying methods is the RAPD technique which has been wildly used in species identification process. Sixteen polymorphic random primers were selected from 120 Operon＇s primers to amplification purposes. The results show that： （1） The average genetic similarity index within two species is 0.8853 in N.japonica and 0.8901 in N. miichthioides. The variances of average genetic similarity between individuals within each species were detected by SPSS software. There was significant difference （P〈0.05）among the species ofN. miichthioides and no such character among the species of N.japonica. （2） The genetic distance between the two species was 0.2150, indicating the N.japonica and N. miichthioides belong to two different species, respectively.

关 键 词：日本黄姑鱼 鮸状黄姑鱼 RAPD标记 遗传变异 

分 类 号：S917[农业科学—水产科学]