Chiralcel OD-RH手性柱拆分安息香对映体的热力学研究  

Thermodynamic Study on the Enantioseparation of Benzoin on a Chiralcel OD-RH Column

作　　者：成芳[1] 邱进坤[1] 阮源萍[1] 

机构地区：[1]厦门大学化学化工学院化学系现代分析科学教育部重点实验室,福建厦门361005

出　　处：《分析科学学报》2007年第3期253-256,共4页Journal of Analytical Science

基　　金：福建省自然科学基金(No.0510001)

摘　　要：利用Chiralcel OD-H手性柱拆分外消旋安息香化合物制备单一对映体标样,并对其进行圆二色光谱表征。在此基础上,采用甲醇、乙醇、乙腈为流动相考察了安息香在Chiralcel OD-RH柱上的色谱拆分行为和热力学特性。结果表明:以纯甲醇为洗脱液时,(S)-构型先出峰,安息香的拆分过程为焓控;以纯乙醇为洗脱液时,(R)-构型先出峰,拆分过程为熵控;而以纯乙腈为洗脱液时,虽然也是(R)-构型先出峰,但熵和焓对拆分均有贡献。文中还对比了安息香在Chiralcel OD-H柱的拆分性能。This paper reports the thermodynamic study on enantioseparation of benzoin on a Chiralcel OD-RH column under the revered-phase mode. The effects of different mobile phases and the column temperature on the retention and enantioseparation were investigated. It was found that （S）-benzoin was first eluted and the enantioseparation was typical enthalpy driven with methanol as eluent, but （R）- benzoin was firsted eluted and the enantioseparation was typical entropy driven with ethanol as eluent. However, when acetonitrile was used as mobile phase, （R）-benzoin was first eluted, but enthalpy and entropy terms were all favorable to enantioseparativity. The comparison of enantioseparation of benzoin was also investigated on a Chiralcel OD-H column.

关 键 词：对映体拆分 色谱热力学 安息香 反相液相色谱 

分 类 号：O657.72[理学—分析化学]