咖啡酸锗与hsDNA相互作用的光谱和电化学研究  

Studies on Interaction of Caffeic Acid Germanium with hsDNA

作　　者：侯琳熙[1] 干宁[1] 童爽[1] 陈妮[1] 陈晓东[2] 徐伟民[1] 

机构地区：[1]宁波市新型功能材料及其制备科学国家重点实验室培育基地宁波大学,浙江宁波315211 [2]南京大学化学系,南京210008

出　　处：《分析科学学报》2007年第3期287-290,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.60508012);浙江省科技攻关项目(No.2006C31040)

摘　　要：通过测量咖啡酸锗(CAA-Ge)的荧光强度,并比较加入鲱鱼精DNA(hsDNA)后的荧光光谱和吸收光谱,以及循环伏安曲线变化,判断两者的作用方式,得到两者的本征结合常数达到5.23×104L.mol-1,且一个CAA-Ge分子可同时与6个hsDNA碱基对发生作用。并通过改变体系,进一步证明了两者的键合方式为嵌入和静电吸引为主的混合方式。To study the interaction between Caffeic Acid Germanium （CAA-Ge） and DNA, fluorescence intensity of CAA-Ge was measured. The fluorescence and absorption spectral along with cyclic voltammetry variations in the absence and presence of hsDNA were also studied. The intrinsic binding constant of CAA-Ge with hsDNA was detemined to be 5.23×10^4 L·mol^-1, and one CAA-Ge molecule could bind to 6 base pairs. The binding mode was proved to be mix mode with intercalation and electrostatic absorption.

关 键 词：咖啡酸锗 鲱鱼精DNA 荧光 相互作用 循环伏安法 

分 类 号：O657.3[理学—分析化学]