可控性微结构磷酸钙支架作为成骨细胞载体修复兔尺骨节段性缺损  

Caldum phosphate ceramic scaffold with controlled internal architecture as osteoblast carrier to rapair a rabbit ulnar critical-size segmental defect model

作　　者：王林[1] 王臻[1] 李祥[2] 李涤尘[2] 卢秉恒[2] 郭征[1] 卢建熙 许宋锋[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032 [2]西安交通大学机械制造系统工程国家重点实验室 [3]法国Littoral大学生物材料和生物技术研究所

出　　处：《中华实验外科杂志》2007年第6期662-664,F0004,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(50235020);上海市科委资助项目(05DJ14005)

摘　　要：目的采用可控性微结构磷酸钙支架作为成骨细胞载体修复兔尺骨节段缺损,观察载体微结构在新骨生成及缺损愈合中的作用并探讨其机制。方法应用快速成型(RP)间接制造技术制备可控性微结构自固化磷酸钙(CPC)支架,将兔颅骨源性成骨细胞与实验组(可控性微结构支架)及对照组(单纯CPC材料)复合培养,倒置显微镜及扫描电镜(SEM)观察细胞生长情况;培养7 d后植入兔尺骨节段性缺损,术后4、8、12周取材,分别行大体、X线、组织学观察和新生骨定量分析,评价新骨生长及缺损愈合情况。结果培养7d后支架表面及管道内有大量细胞分布。实验组新骨生长、改建及材料降解均沿管道结构进行,术后12周形成新生骨与支架相互嵌合的复合体;对照组新骨生长主要发生在骨断端与材料结合处,术后12周两端骨组织长入材料,CPC出现少量降解。术后12周实验组新生骨量高于对照组,差异有统计学意义(P<0.05)。结论可控性微结构促进支架内新骨生成及兔尺骨节段性缺损的愈合,对支架微结构的深入研究和优选是增强组织工程化骨移植物成骨效能的有效途径。Objective To evaluate the effect of calcium phosphate cement （CPC） scaffold with controlled internal architecture as osteoblast carrier on bone response in a rabbit ulnar defect model. Methods The self-hardening CPC scaffolds were fabricated using indirect rapid prototyping （RP） and computer aided design （CAD） techniques. Osteoblasts were isolated from skull of rabbits and seeded onto CPC scaffolds with or without controlled internal channel architectures. Some constructs were observed under a phase-contrast microscope and scanning electron microscope （SEM） and others were transplanted into 1.5 cm rabbit ulnar defect after cultured in vitro for 7 days. The specimens harvested at 4,8, and 1：2 weeks after operation respectively were evaluated by gross observation, X-ray examination, histology staining, and image pattern analysis. Results Under SEM after 7 days of incubation,confluent cell layer was observed on scaffold surface and in channels. New bone proliferation, reconstruction, and CPC degradation were observed along the controlled internal channels in scaffold and a composite of new bone and scaffold was found at 12th week after operation. Primary new bone generation was found between defect margins and the CPC scaffold without channel architectures. Bone proliferation was found from defect margins into CPC and a smal/quantity of material degradation was observed at 12th week. Scaffolds with controlled channel architectures had higher percentages of new bone area compared to scaffolds without engineered channels （P 〈 0.05）. Conclusion The controlled scaffold architecture can enhance the ability of a ceramic material to accelerate healing of rabbit ulnar defect.

关 键 词：骨缺损 移植 支架 磷酸钙 

分 类 号：R687[医药卫生—骨科学]