海藻糖在同种瓣低温保存中的保护作用  

Cryoprotective effect of trehalose in aortic valve homograft

作　　者：常青[1] 徐平[1] 李守先[2] 

机构地区：[1]青岛大学医学院附属医院心血管外科,266003 [2]山东大学齐鲁医院心血管外科

出　　处：《中华实验外科杂志》2007年第6期706-708,共3页Chinese Journal of Experimental Surgery

基　　金：青岛市科技发展计划资助项目(k2d-03)

摘　　要：目的探讨非渗透性低温保护剂海藻糖联合应用二甲基亚砜,用于同种瓣的深低温储存,并与常规应用的二甲基亚砜的冻存效果进行比较。方法分别以10%二甲基亚砜+0.1 mol/L海藻糖(实验组)和10%二甲基亚砜(对照组)作为冷冻保护剂,经程控梯度降温,液氮冻存大鼠的同种瓣6个月后复温,电镜、光镜观察、内皮细胞活力及葡萄糖代谢率测定,比较同种瓣的组织活性、代谢功能和结构变化。结果实验组的葡萄糖代谢率为(20.570±1.789)g/,L·d^(-1)明显高于对照组(18.621±1.842)g/L·d^(-1)(P<0.01),内皮细胞活力(77.75±6.60)%也明显高于对照组(69.81±4.40)%(P<0.01),并且其结构的破坏也较对照组轻(P<0.05)。结论10%二甲基亚砜+0.1 mol/L海藻糖作为同种瓣的冷冻保护剂,其保护效果明显优于10%二甲基亚砜。Objective To search the best cryoprotectant for preserving aortic valve homograft in liquid nitrogen. Methods Cryoprotectant in test group ： 10% DMSO ＋ 0.1 mol/L trehalose; Cryoprotectant in control group ：10% DMSO. The aortic valve homografts of Wistar rats were gradually hypothermied and then cryopreserved in 196℃ liquid nitrogen. After 6 months, all the cryopreserved valves were thawed by the method of immersing in 40℃ saline pool. The activity of cryopreserved valve was measured by glucose utilization and the viability of endotheliocyte. At the same time ,the structure of cryopreserved valve was observed by light .microscope and electron microscope. Results The viability of endotheliocyte and tissue glucose consumption in test group were significantly higher than in control group, while the destruction of structure in test group was milder than in control group. Conclusion The effect of the cryopmtectant which was composed of 10% DMSO ＋ 0.1 mol/L trehalose was much better than that of the cryoprotectant composed of 10% DMSO.

关 键 词：瓣 二甲基亚砜 海藻糖 葡萄糖 

分 类 号：R654.2[医药卫生—外科学]