骨髓间充质干细胞输对移植肾的保护作用  

Protective effect of bone marrow-derived mesenchymal stem cells infusion on the renal grafts

作　　者：周云峰[1] 张炜[1] 张炜小[1] 秦超[1] 吴婷[1] 

机构地区：[1]南京医科大学第一附属医院泌尿外科,210029

出　　处：《中华实验外科杂志》2007年第6期721-723,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省科技厅重大招标资助项目(BK2004004)

摘　　要：目的体外培养大鼠骨髓间充质干细胞(MSCs),探讨其对移植肾的保护作用。方法无菌条件下取大鼠股骨、胫骨,用直接贴壁法分离纯化MSCs,体外扩增,在注射MSCs的基础上,行同种异体肾移植,实验大鼠分为注射大鼠骨髓MSCs肾移植组(Ⅰ组)和单纯肾移植组(Ⅱ组),环孢素A治疗肾移植组(Ⅲ组)术后5 d结扎受体右肾动脉(左侧为移植肾);观察右肾动脉结扎术后受体存活时间,检测右肾术后移植肾的形态学变化,肌酐水平。结果Ⅰ组、Ⅲ组大鼠生存期(15.50±6.35)d和(18.50±5.97)d较Ⅱ组(6.50±3.11)d明显延长,且前者移植肾形态学观察和功能状态均明显优于后者。结论骨髓间充质干细胞在体外很容易分离培养和扩增,骨髓间充质干细胞对早期移植肾具有保护作用。Objective To culture rat mesenchymal stem cells （MSCs） and explore the protective effects of MSCs on the renal grafts. Methods Bone marrow was collected from SD rats by flushing femurs and tibias under sterile condition. MSCs were purified by density gradient centrifugation or stillness-stickcultivation in vitro. Sixty Wistar rats were transplanted with the left kidneys of SD rats and divided into following groups （n =20 in each group） ：MSCs group （group Ⅰ ） ,control group （group Ⅱ ） and cyclosporine group （ group Ⅲ ）. The survival time and morphology of the renal grafts were observed. Results MSCs that were in low abundance grew quickly when cultured in vitro. The survival time of group Ⅰ [ （ 15.50 d ± 6.35） days ] and group Ⅲ [ （ 18.50 ± 5.97） days ] were longer than in group Ⅱ [ （6.50 ± 3.11 ） days ]. In the groups Ⅰ and Ⅲ, the changes of the renal grafts were milder than in group Ⅲ. Conclusion MSCs can be isolated,cultured and expanded easily in vitro,and have protective effect on the renal graft in early period.

关 键 词：骨髓 间充质干细胞 肾移植 免疫抑制 

分 类 号：R699.2[医药卫生—泌尿科学]