HPLC法测定血浆中盐酸曲马朵药物浓度  

Study on determination of tramadol HCl in plasma by HPLC

作　　者：王丽[1] 李宗明[1] 

机构地区：[1]天津市中医医院,天津300140

出　　处：《天津药学》2007年第3期25-26,共2页Tianjin Pharmacy

摘　　要：目的：建立测定盐酸曲马朵血浆药物浓度的HPLC方法。方法：以非那西丁为内标，正己烷-乙酸乙酯（4：1）在碱性条件下提取血样中曲马朵，流动相为乙腈一KH2PO4（31：69），色谱柱为迪马C18柱，测定波长为218nm。结果：盐酸曲马朵在10—1000ng／nd范围内，标准曲线方程为Y=0．001392X-0．007136；提取血样中曲马朵平均回收率为（90．4±4．2）％；测定的日内和日问相对标准差（RSD）分别为3．0％-6．3％和4．4％-5．5％；室温（25℃）放置8h和冻融3次对血样中盐酸曲马朵测定没有明显影响。结论：本方法灵敏、准确、重复性好，可应用于含盐酸曲马朵制剂的临床药代动力学研究。OBJECTIVE To establish a HPLC method for determination of tramadol in plasma. Method ： Plasma samples （ with phenacetin as internal standard ） were extracted with n - hexane/acetic ether （ 4 ： 1 ） in alkaline condition and determining by HPLC. The mobile phase was composed of acetonitrile：buffer（ 25 mmol/L KH2PO4 containing 0.15% triethylamine） = 31：69 （ V/V）. The chromatographic procedure was carried out using Diamonsil C18 column（4.6 mm × 250 mm, 5 μm） and detection wavelength was at 218 nm. RESULT Tramadol HC1 concentration range was between 10 and 1 000 ng/ml, the average recoveries of tramadol HC1 were （90.4 ± 4.2 ） %. RSD of within day and between days were 3.0% - 6.3% and 4.4% - 5.5%. It had not significant influence that samples were stored in 25 ℃ for 8 h or freeze thawing three times. CONCLUSION The method was a sensitive, precise and repeatable. It could be applied in clinic pharmacokinetics.

关 键 词：高效液相色谱法 盐酸曲马朵 血药浓度 

分 类 号：R97[医药卫生—药品]