上海四膜虫接合生殖期间皮层细胞骨架蛋白的研究  

STUDIES ON PROTEINS OF CORTICAL CYTOSKELETON OF TETRAHYMENA SHANGHAIENSIS DURING CONJUGATION

作　　者：王康乐 庞延斌[2] 

机构地区：[1]南通师专生物系 [2]华东师范大学生物系,上海200062

出　　处：《水生生物学报》1997年第1期8-14,共7页Acta Hydrobiologica Sinica

摘　　要：本文采用生化抽提，结合电泳及显微技术，对上海四膜虫（Tetrahymenashanghaiensis）S1皮层细胞骨架（corticalcytoskeleton）的蛋白组份，及其在接合生殖期间的变化进行了一系列的研究。初次探索了S1株上海四膜虫在接合生殖过程中皮层细胞骨架的蛋白组份及含量，并分析了它们与间期、接合分开时期同类蛋白相互间的差异。发现在接合生殖时期76－88kD蛋白有突出的表现，而90kD和85kD特异性地存在于接合生殖期间，它们与接合区膜融合的进一步稳固，小核的减数分裂过程有某种联系。Tetrahymena shanghaiensis S1 is a selfer strain. By starvation, more than 85% of the cells in a clone can be induced to conjugation synchronously. Using biochemical extraction, SDS-PAGE, combining with microscopic examination, we studied the major components and contents of cortical cytoskeleton protein of T.shanghaiensis S1, and explored the extraordinary changes in these proteins during conjugation for the first time.The similarity and difference between cortical cytoskeletal proteins of vegetation, conjugation and exconjugation cells are compared in this paper.The results of SDS-PAGE and protein isolation showed that the essential component of all cortical cytoskeleton proteins (225kD, 150kD、 120kD、52.5kD, 49kD、37-43kD) decreased during and after conjugation. We also observed great fluctuation of the 76-88kD proteins of the oral apparatus in different cell phases.Two particular bands are obvious: one is the 90kD protein and the other one is 85kD protein, both can be found after membrane fusion and during meiosis We recognice that during the conjugation proteins change obviously in all cortical cytoskeleton of T.shanghaiensis.

关 键 词：上海四膜虫 接合生殖 皮层细胞 骨架蛋白 

分 类 号：Q959.117.3[生物学—动物学]