萝卜雄性不育系花药发育组织化学的初步研究  

HISTOCHEMICAL STUDIES ON ANTHER DEVELOPMENT IN MALE STERILE RADISH (RAPHANUS SATIVUS L.)

作　　者：董庆华[1] 利容千[1] 王建波[1] 

机构地区：[1]武汉大学生命科学学院,武汉430072

出　　处：《武汉植物学研究》1997年第1期10-14,共5页Journal of Wuhan Botanical Research

基　　金：国家教委高等学校博士点基金

摘　　要：对萝卜（RaphanussativusL．）2种雄性不育系及相应保持系花药发育过程进行组织化学研究。研究结果表明，2种不育系花药组织化学变化与小孢子败育变化皆类似。不育系475A的花药发育受阻于泡原细胞分化之前，此时花药中含少量蛋白质、核酸和较多淀粉粒。随着花药的长大，不再含有蛋白质、核酸和淀粉粒。不育系春红A的花药在四分体以前与可育系类似，含丰富蛋白质、核酸和多糖。在单核期绒毡层异常引起小抱子败育的过程中，花药中蛋白质、核酸和多糖含量迅速减少，不再含有淀粉粒。Histochemical studies were conducted on the anther development of two male-sterile lines and their fertile line in radish. The histochemical changes of male-sterile lines concided with the morphological changes. The inhibition of 475A anther development occurred before the stage of archesporial cell. At that time,the anther possessed little protein and nucleic acids, though some starch grains were observed. With the growth of anther, no protein,nucleic acids and starch grains were observed. Before the tetrad stage,the Spring Red A was normal and stored rich polysaccharides,protein and nucleic acids. At the stage of nuinucleate microspore,the tapetal cells developed abnormally. During the abortion of pollen,the protein,polysaccarides and nucleic acids disappeared rapidly,and no starch grains were observed.

关 键 词：萝卜 雄性不育 花药 发育 组织化学 

分 类 号：S631.101[农业科学—蔬菜学]