乙酰肝素酶在大鼠酒精肝纤维化中的表达及其意义  

The expression of heparanase and its significance in alcoholic hepatic fibrosis of rats

作　　者：洪洋懿[1] 阳学风[2] 雷军[3] 王海林[4] 

机构地区：[1]广东省广州市花都区人民医院,广东广州510800 [2]南华大学第二附属医院消化内科,湖南衡阳421000 [3]广东省广州市花都区人民医院ICU病房,广东广州510800 [4]广东省广州市第一人民医院放射科,广东广州510180

出　　处：《世界感染杂志》2007年第3期207-210,共4页World Journal of Infection

摘　　要：目的观察乙酰肝素酶（HPSE）在酒精诱导的大鼠肝纤维化时的动态表达，以探讨其在肝纤维化形成过程中的作用。方法采用酒精-玉米油-吡唑灌胃诱导肝纤维化模型，分别用免疫组织化学染色．和原位杂交方法检测乙酰肝素酶的蛋白和mRNA在大鼠酒精性肝纤维化发生发展中的动态表达。HE、Masson染色判断肝纤维化程度。结果乙酰肝素酶在酒精性肝纤维化组表达阳性率明显高于正常肝组织（8／17vs0／11，P〈0．005），有显著的统计学差异。乙酰肝素酶的蛋白、mRNA表达模式和定位基本一致。结论乙酰肝素酶参与了早期肝纤维化的形成和发展，且乙酰肝素酶的合成与储存在同一细胞。Objective To explore the significance of heparanase in rat hepatic fibrosis induced by alcohol. Methods In rat models of alcoholic fibrosis, the expression of heparanase mRNA and protein were detected by in situ hybridization and by immunohistochistochemistry. The tissue specimens were stained with HE and Masson methods to evaluate the grade of liver fibrosis. Result The expression of heparanase mRNA and protein were up-regulated in the alcoholic hepatic fibrosis and higher than that of normal liver tissues （8/17 vs 0/11, P〈0.005）. Moreover, a similar pattern of expression was observed both at mRNA and protein level. Conclusions It can be concluded that heparanase is involved in the pathogenesis of liver fibrosis. In addition, the co-expression of heparanase mRNA and protein implies that heparanase is synthesized and stored in the same ceil.

关 键 词：乙酰肝素酶 酒精性肝纤维化 免疫组化 原位杂交 

分 类 号：R657.31[医药卫生—外科学] R-332[医药卫生—临床医学]