培养条件对钝顶螺旋藻(Sp)NS-90020脂肪酸组成和含量的影响  

EFFECTS OF CULTURAL CONDITIONS ON FATTY ACID COMPOSITION AND CONTENT IN SPIRULINA PLATENSIS

作　　者：殷春涛[1] 胡鸿钧[1] 龚小敏[1] 耿亚红[1] 

机构地区：[1]中国科学院武汉植物研究所,武汉430074

出　　处：《武汉植物学研究》1997年第1期59-65,共7页Journal of Wuhan Botanical Research

摘　　要：研究了不同培养条件对钝顶螺旋藻（Sp）NS－90020脂肪酸合成的影响。随着温度升高，其不饱和脂肪酸，γ一亚麻酸（GLA）相对含量降低，总脂肪酸含量升高，当温度为40℃时总脂肪酸和γ-亚麻酸绝对含量都达到最大值，分别为73．4mg／g干重和11．9mg／g干重；当培养基中NaCl浓度高于0．017mol／L时，其GLA相对含量降低，但低于0．0017mol／L时，对其脂肪酸组成无显著影响；氨水使其脂肪酸和GLA绝对含量升高，并在50mgN（NH3·H2O）时达到最大值，分别为67．96mg／g干重和13．63mg／g千重；暗处理92h使其总脂肪酸和GLA绝对含量升高；缺乏Fe2+或Mg2+或Mo2+时，其总脂肪酸和GLA绝对含量降低，而缺乏PO43-时，其总脂肪酸和GLA绝对含量略有升高。Effects of cultural conditions on fatty acid composition and content in Spirulina platensis (SP)NS-90020 were studied. With increasing temperature, the relative contents of polyunsaturated fatty acid and GLA in (Sp)NS-90020 decreased,but the content of total fatty acid increased,the absolute contents of total fatty acid and GLA reached the highest level at 40'C,and they were 73. 4 mg/g DW, 11. 9 mg/g DW, respectively.When NaCI concentration was higher than 0. 001 7 mol/L in the medium, the relative content of GLA decreased,but lower than 0. 001 7 mol/L had no effect on the fatty acid composition. The absolute contents of total fatty acid and GLA increased in the presence of amonia,and their highest contents were obtained when amonia was added at the level of 50 mg N(NH,. H,O),they were 67. 96 mg/g DW, 13. 63 mg/g DW,respectively. The absolute contents 6f total fatty acid and GLA increased under 92 hours dark treatment during the exponential phase. The absolute contents of total fatty acid and GLA reduced when Fe2+, Mg2+ or Mo2+ was deficient in the medium,but PO43 defienciency elevated the absolute contents of total fatty acid and GLA.

关 键 词：钝顶螺旋藻 培养条件 Γ-亚麻酸 脂肪酸 

分 类 号：Q949.220.6[生物学—植物学]