介绍一种测定溶血的高灵敏度方法  

Recommendation of a Highly Sensitive Method for Measuring Hemoglobin in Hemolytic Test

作　　者：胡望平[1] 胡盈莹[2] 冯福英[1] 黄兢姚[3] 张荣盛[1] 陈荣仁[1] 周长邵[1] 王海林[1] 

机构地区：[1]南京军区福州总医院生化科,福建医科大学福总临床医学院,福州350025 [2]福州市传染病医院肝病研究所,福州350025 [3]福建医科大学协和医院,福州350025

出　　处：《生物医学工程学杂志》2007年第3期664-666,共3页Journal of Biomedical Engineering

摘　　要：建立灵敏度高、试剂安全测定血浆游离血红蛋白(FHb)的方法。用于各项溶血试验。其方法为设计改良Trinder反应的2,4-二氯酚(2,4-DCP)方法,并进行各项性能验证。结果显示:本法灵敏度是酚法的2.39倍,批内批间重复性好。呈色稳定,FHb参考值为1～36.7mg/L,以液体枸橼酸钠抗凝剂,保存红细胞最优。FHb2,4-二氯酚法无毒、无癌原性,适用临床及生物医学工程学的各项溶血试验。In this paper is recommended a highly sensitive and reagent-safe method to determine plasma heamoglobin （FHb） in viscacha hemolytic test. The 2,4-dichlorophenol method （2,4-DCP） of Trinder reaction has been improved. The performance of 2,4-DCP is verified. The sensitivity of 2,4-DCP is 2.39 times that of phenol method. It is well used with run precision and day-to-day precision. The reaction color is stable. The reference value FHb is 1-36.7 mg/L. Sodium citric is an excellent anticoagulant liquid to keep erythrocyte. The 2,4-DCP method is neither carcinogenic nor poisonous;it is suitable for viscacha hemolytic test in clinical and biomedical engineering.

关 键 词：溶血试验 血浆血红蛋白 TRINDER反应 体外循环 2 4-二氯酚法 

分 类 号：R318[医药卫生—生物医学工程]