一株异养硝化菌的筛选及其脱氮条件  

Screening of a heterotrophic nitrifying bacterium strain and its optimal conditions for nitrogen removing

作　　者：陈赵芳[1] 尹立红[1] 浦跃朴[1] 李先宁[2] 谢祥峰[2] 

机构地区：[1]东南大学公共卫生学院,南京210009 [2]东南大学能源与环境学院,南京210096

出　　处：《东南大学学报（自然科学版）》2007年第3期486-490,共5页Journal of Southeast University：Natural Science Edition

基　　金：江苏省自然科学基金资助项目(BK2005068;BK2004075);高等学校博士学科点专项科研基金资助项目(20050286035)

摘　　要：从生活污水生物脱氮除磷装置中分离到一株脱氮效果较好的异养硝化菌株,脱氮过程中无亚硝酸盐氮积累质量,只有少量硝酸盐氮积累.在实验室条件下,初步探讨了不同温度、pH值、摇床转速、碳氮比、氨氮质量浓度对YY4菌株脱氮作用的影响.研究结果显示:温度为30℃、pH值为9.0、摇床转速150r/min、m(C)/m(N)为10、氨氮质量浓度为100mg/L时,YY4菌株具有最佳的脱氮效果.应用该菌株对宜兴生活污水和南京某化工厂废水氨氮脱除效果的结果显示,去除率分别为89.54%(9h)和95.79%(36h).A heterotrophic nitrifying bacterium with low nitrate and no nitrite accumulation during denitrifying process was isolated from sewage disposal. The effects of temperature, pH, shaking speed, m（ C）/m（N） and concentration of NH4 -N on denitrification of YY4 bacterium were investigated. The results show that the optimal conditions for NH4^＋ -N removing are as follows： temperature 30℃, pH 9.0, shaking speed 150 r/min, m（C）/m（N） 10, NH4^＋-N 100 mg/L. The obtained strain can achieve a NH4 ^＋-N removing rate of 89.54% from domestic sewage after 9 h and 95.79% from industrial wastewater after 36 h.

关 键 词：异养硝化菌株 脱氮条件 脱氮作用 

分 类 号：X703.1[环境科学与工程—环境工程]