核因子-κB在急性坏死性胰腺炎大鼠肺损伤中的作用  

Effect of Nuclear Factor Kappa B on Lung Injury of Rats with Acute Necrotic Pancreatitis

作　　者：崔勇[1] 龙泽民[1] 刘任飞 刘瑾琨[3] 

机构地区：[1]深圳市蛇口人民医院普外科,广东深圳518067 [2]武警吉林总队第一支队卫生队 [3]深圳市第二人民医院普外科

出　　处：《中国实验诊断学》2007年第6期734-735,共2页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨核因子-κB(NF-κB)在急性坏死性胰腺炎大鼠肺损伤发病中的作用。方法制作大鼠急性坏死性胰腺炎(ANP)模型,随机分为正常对照组、急性坏死性胰腺炎组。应用原位杂交法检测肺组织NF-κB p65mRNA的表达。结果ANP组肺组织病理改变明显;NF-κB p65mRNA在ANP时除肺泡的细胞质内有表达外,出现细胞核内表达,随时间的延长表达逐渐增加。结论NF-κB活化是急性坏死性胰腺炎早期细胞内重要事件,NF-κB与ANP的肺损伤密切相关。Objective To investigate the effect of nuclear factor kappa B （NF-κB） on lung injlury of rots with acute necrotic panereatitis （ANP）.Methods Wistar rats were inflicted with acute necrosis panereatitis and randomized equally into the eontrol group, acute necrosis pancreatitis group. The expression of NF-κB p65mRNA in lung tissues were deterrrdned by in situ hybridization. Restflts Pathologic change were evident in AN7 groups,Tile expression of NF-κp65mlRNA were determined in karyon and cytoplasm. Conclusion NF-κaetivation is the important event in cell of acute necrotic panereatitis, NF-κmay plays an important role in lung injury associated with ANP.

关 键 词：胰腺炎 核因子-ΚB 肺损伤 

分 类 号：R657.5[医药卫生—外科学]