布鲁氏菌pcDNA3.1-omp17.3基因疫苗的研制  

Development of DNA vaccine pcDNA3.1-omp17.3 against Brucella

作　　者：邓小红[1] 曾政[2] 王希良[3] 

机构地区：[1]重庆工商大学药物化学与化学生物学研究中心,重庆400067 [2]重庆市动物疫病预防控制中心,重庆401147 [3]军事医学科学院微生物流行病研究所,北京100071

出　　处：《中国兽医科学》2007年第6期505-509,共5页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30170853)

摘　　要：采用PCR方法扩增了布鲁氏菌17.3 ku外膜蛋白编码基因,并将该基因克隆至真核表达载体pcDNA3.1(+)中,成功构建了真核表达质粒pcDNA3.1-omp17.3。pcDNA3.1-omp17.3转染COS-7细胞后,通过Western-blotting检测到了17.3 ku蛋白的瞬时表达。将pcDNA3.1-omp17.3免疫小鼠,三免后经ELISA、流式细胞仪以及ELISPOT技术检测到pcDNA3.1-omp17.3在小鼠体内诱导产生了以Th1型为主的细胞免疫应答。结果表明,构建的基因疫苗可作为潜在的布鲁氏菌新型疫苗,有进一步研究的意义。The ompl7.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3. 1- omplT. 3. Then the recombinant plasmid pcDNA3. 1-omplT. 3 was transfected into COS7 cells, and the expressed OMP 17. 3 was detected by Western-blotting. The recombinant plasmid was injected into BALB/c mice intramuscularly. The immunized mice were induced to produce a typical T helper 1 dominated immune response, as determined by ELISA, Flow Cytometer and ELISPOT method. The results Showed that pcDNA3.1-omp17. 3 was a potential candidate vaccine for the controlling of brucellosis.

关 键 词：布鲁氏菌 17.3 KU蛋白 基因疫苗 免疫应答 

分 类 号：S852.614[农业科学—基础兽医学]