表达霍乱CT-B和LPS-O抗原的鼠伤寒菌苗株的构建  

Construction of A Salmonella typhimurium Vaccine Strain Expressing Vibrio cholerae CT B and LPS O Antigen

作　　者：陈东立[1] 马清钧[1] 

机构地区：[1]军事医学科学院生物工程研究所

出　　处：《生物工程学报》1997年第1期47-52,共6页Chinese Journal of Biotechnology

摘　　要：将编码霍乱ＣＴＢ和ＬＰＳＯ抗原的基因与载体质粒ｐＹＡ２４８重组后，转入ΔｃｙａΔｃｒｐΔａｓｄ减毒鼠伤寒疫苗株ｘ４０７２，构建成无药物抗性、带双价抗原的工程菌株ｘ４０７２（ｐＭＧ３０６）。该菌株能分泌表达特异的霍乱ＣＴＢ抗原，并且在菌体表面同时表达霍乱和鼠伤寒的Ｏ抗原。小鼠腹腔免疫和攻击试验表明该菌苗株对霍乱毒株的攻击具有良好的保护作用。The genes encoding V.cholerae CT B and LPS O antigen were simultaneously inserted into vector plasmid pYA248. The resulting recombinant plasmid pMG306 was transformed into Δ cya Δ crp Δ asd attenuated S.typhimurium vaccine strain x4072,and the live vaccine strain x4072(pMG306) was constructed.This vaccine strain could secrete specific CT B antigen. Meanwhile,LPS O antigens of both V.cholerae and S.typhimurium were expressed on the cell surface. Mouse intraperitoneal immunization and challenge trial indicated x4072(pMG306) provided good protection against virulent V.cholerae. This study laid foundation of the development of new cholera typhoid bivalent live oral vaccine.

关 键 词：霍乱弧菌 CT-B抗原 LPS-O抗原 鼠伤寒杆菌 疫苗 

分 类 号：R378.23[医药卫生—病原生物学] R378.3[医药卫生—基础医学]