花生根瘤菌的生长特性和脂肪酸组成研究(英文)  

GROWTH CHARACTERISTICS AND FATTY ACID ANALYSES OF BRADYRHIZOBIUM STRAINS ISOLATED FROM THE ROOT NODULES OF PEANUT(ARACHIS)

作　　者：张小平[1] 李登煜[1] K.Lindstrom G.Nick S.W.Tighe 

机构地区：[1]四川农业大学农学院应用微生物系 [2]AnalyticalServicesInc,POB626,EssexJunction,VT0543,USA [3]DepartmentofAppliedChemistryandMicrobiology,BiocenterIA,POB27,00014,UniverstiyofHelsinki,Finland

出　　处：《应用与环境生物学报》1997年第1期44-48,共5页Chinese Journal of Applied and Environmental Biology

基　　金：联合国科教文组织生物工程基金;国家自然科学基金;赫尔辛基大学资助

摘　　要：用73株慢生根瘤菌B．Japonicum、B．elkanii和B．"intermedium"为对照．对22株花生根瘤菌的生长速度、产碱能力和细胞脂肪酸组成进行了测定．结果表明：花生根瘤菌属于慢生根瘤菌属（Bradyrhizobiumsp．arachis）；除花生根瘤菌含有比大豆根瘤菌（B．japonicum）较高的脂肪酸16：1w5c（16个碳原子1个双键的脂肪酸）而外，花生根瘤菌与大豆根疚菌相似，表明了他们在系统发育及进化方向上的一致性．细胞脂肪酸分析法是根瘤菌系统发育研究中一种简便而可信的方法．Twenty two rhizobial strains isolated from the root noduled of two Chinese peanut cultivars (Arachis hypogaesa L. Tianfu no. 3 and a local cultivar) growing in four different areas in Sichuan were analyzed and compared with other bradyrhizobial strains form strains from Bradyrhizobium japonicum, B. alkanii and B. intermedium.The results showed that all the 22 rhizobial isolates from the peanut in Sichuan were bradyrhizobia. FAME and two-dimensional analyses on the principal component indicated component indictted that Bradyrhizobium sp. (arachis) was dif ferent from other bradyrhizobia because of the presence of fatty acid 16:1 waid with significantly higher perecntage, but closely related to B. japonicum. The results suggest that resluts suggest that FAME analysis that FAME analysis provides a relatively simple and reliable procedure for the inital characterization of Bradyrhizobium isolates.

关 键 词：花生根瘤菌 脂肪酸 生长特性 

分 类 号：Q939.114[生物学—微生物学]