不同鼠疫检测技术在河北省鼠疫监测中的应用研究  

Study on application with different testing technologies of plague surveillance in Hebei province

作　　者：史献明[1] 刘合智[1] 杜国义[1] 白雪薇[1] 王海峰[1] 张月枝[1] 杨建民 李贵民 

机构地区：[1]河北省鼠疫防治所,张家口075000 [2]康保县疾病预防控制中心 [3]围场县疾病预防控制中心

出　　处：《中国媒介生物学及控制杂志》2007年第3期222-225,共4页Chinese Journal of Vector Biology and Control

基　　金：国家"十五"重点攻关资助项目(2004BA718B07-01)

摘　　要：目的通过用鼠疫血凝试验、胶体金标记免疫层析(金标)和ELISA 3种方法检测啮齿动物血清,用金标、ELISA、PCR与鼠疫反向血凝试验和细菌分离检测啮齿动物脏器,评价其在鼠疫监测中的应用效果。方法用鼠疫血凝试验、金标和ELISA 3种方法检测啮齿动物血清;用金标、ELISA、PCR、鼠疫反向血凝试验和细菌分离检测啮齿动物脏器。结果鼠疫血凝试验、金标和ELISA 3种方法对啮齿动物血清的阳性检出率分别为0.32%、0.32%和1.26%。金标、ELISA、PCR、鼠疫反向血凝试验和细菌分离对啮齿动物脏器的阳性检出率分别为3.15%、0.70%、4.90%、0.70%和0.70%。结论金标、ELISA、PCR值得推广应用,非常适合基层和快速诊断应用。Objective To value application effects on plague surveillance using different methods. Methods Rodent serums were detected by indirect hemagglutination assay（IHA）, gold-immunochromatography assay（GICA-Ag）, enzymelinked immunosorbent assay （ELISA-Ag）. Rodent viscera were detected by gold-immunochromatography assay （GICA-Ab）, enzymelinked immunosorbent assay （ ELISA-Ab ）, polymerase chain reaction （ PCR ）, bacterial isolation, reverse indirect hemagglutination assay（RIHA）. Results Antibody detection rates in serums using IHA, GICA-Ag, ELISA-Ag were 0.32%, 0.32%, 1.26%, respectively. Positive detection rates of GICA-Ab, ELISA-Ab, PCR, RIHA, bacterial isolation in rodent viscera were3.15%, 0.70%, 4.90%, 0.70%, 0.70%, respectively. Conclusion GICA, ELISAand PCR are valueable to extend and apply. They are suitable to use in basic level and fast diagnosis.

关 键 词：胶体金标记免疫层析 酶联免疫吸附试验 聚合酶链反应 鼠疫监测 应用 

分 类 号：R254.8[医药卫生—中医内科学]