四个不同来源途径玉米自交系的DNA指纹分析  

The Analysis of DNA Fingerprints in the four Series Maize Inbred from Different Source

作　　者：陈杰[1] 郑根昌[1] 荆彦辉[1] 马志强[1] 郑毅[1] 

机构地区：[1]内蒙古民族大学,内蒙古通辽028043

出　　处：《内蒙古民族大学学报（自然科学版）》2007年第3期289-292,共4页Journal of Inner Mongolia Minzu University：Natural Sciences

摘　　要：利用SSR分子标记技术,对不同来源的四个玉米自交系65232、Mo17、P138、917进行DNA指纹检测.检测了75对引物,其中Bnlg1439、Bnlg197、Phi126等8对在谱带位置或数量上已出现差异.分析表明,自交系在使用过程中已发生分化或遗传漂移,在自交系提纯复壮中可以利用这8个标记进行辅助选择育种.The study on DNA fingerprinting of four series maize inbred from different source which were 65232, Mo17, P138,917 by using SSR technique. 75 pairs of primers were tested and eight of them were found some changes of PCR bands in quantity and position, such as Bnlg1439, Bnlg197,Phi126. The experiment results indicated that these inbred lines had been involved or haven Genetic drift. So we carry purification and rejuvenation of maize inbred, we can use these 8 pairs SSR markers in molecular marker- assisted selection.

关 键 词：SSR 玉米自交系 DNA指纹 

分 类 号：S338[农业科学—作物遗传育种]